吉亚迪亚腹盘的圆顶结构对于附着和宿主病原发生是必要的
bioRxiv : the preprint server for biology
|July 18, 2023
概括
这项研究表明,Giardia lamblia腹盘蛋白MBP对于形成寄生虫的附着器官至关重要. 破坏MBP可以防止Giardia破坏宿主细胞并导致肠道屏障崩.
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 胃肠病学 胃肠病学
背景情况:
- 吉亚迪亚兰布利亚菌通过独特的腹腔盘附着在宿主肠道上.
- 这种附着被认为会损害宿主细胞并破坏肠道屏障的完整性.
- 腹盘具有复杂的结构,包括微管聚合物和蛋白质复合物.
研究的目的:
- 调查腹盘蛋白MBP在Giardia附着和宿主细胞损伤中的作用.
- 创建和描述一个缺乏功能MBP的Giardia突变体.
- 用一种有机体模型评估受损附着对病原发生的影响.
主要方法:
- 通过CRISPR介导的基因干扰被用于在Giardia lamblia中创建MBP基因的四倍淘汰.
- 分析了MBP淘汰赛 (KO) 突变体的形态学和附着能力.
- 使用胃肠道器官模型来比较野生类型和MBP KO Giardia感染的病原性.
主要成果:
- 麻突变的MBP突变者表现出一个平坦的腹盘,缺少其特有的圆顶形状,并且在微带交桥复合体中存在缺陷.
- MBP KO突变体无法抵抗液体流动,这表明附着功能受损.
- 在有机体模型中,感染MBP KO突变体的感染显示出显著降低了引起上皮质屏障破坏的能力.
结论:
- 在Giardia lamblia中,MBP对于圆顶腹盘结构的形成至关重要.
- 寄生虫的附着,由腹盘介导,是直接需要引起宿主上皮质屏障的破坏.
- 这项研究提供了第一个直接证据,将Giardia附着机制与宿主病理联系起来.
更多相关视频
07:00Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Published on: August 5, 2021
3.4K
09:56Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
Published on: August 12, 2010
16.3K
相关概念视频
Diversity of Protists I
38
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
38
Diversity of Protists II
50
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
50
Fungal Phylum Microsporidia
42
Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...
42
Adherens Junctions
4.9K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.9K
Diversity of Protists IV
40
Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
40
Mucosal Barrier of the Stomach
718
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
718
