相关实验视频
Updated: Jul 23, 2025

09:05
MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
7.6K
阿尔戈纳特的核定位受细胞密度的影响,可能缓解微RNAs的抑制
Krystal C Johnson1, Audrius Kilikevicius2, Cristina Hofman1
1UT Southwestern Medical Center, Departments of Pharmacology and Biochemistry, Dallas, TX 75235.
bioRxiv : the preprint server for biology
|July 18, 2023
概括
在特定条件下,阿尔戈诺特蛋白2 (AGO2) 移动到细胞核,去抑制基因点并增加细胞迁移. 这种核转移受到miRNA水平和RNAi因素的影响.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 细胞生物学 细胞生物学
背景情况:
- 阿尔戈纳特蛋白 (AGO) 是通过微RNA诱导的沉默复合体 (miRISC) 进行转录后基因沉默的关键.
- 通常是细胞质的AGO定位对于miRNA功能至关重要,但核积累及其后果仍然不清楚.
- 环境和细胞条件可以诱导AGO蛋白的核定位.
研究的目的:
- 为了研究阿尔戈诺特蛋白2 (AGO2) 核定位的后果.
- 确定细胞条件和RNAi因素如何影响AGO2局部化和miRNA活动.
主要方法:
- 在不同培养密度的HCT116细胞和3D瘤球形中观察到AGO2局部化.
- 在人类结肠瘤中分析了AGO2局部化.
- 利用AGO2.2的工程核定位信号.
- 研究了淘汰DROSHA和TNRC6对AGO2局部化的影响.
主要成果:
- 在高密度2D培养物,3D瘤球体和人类结肠瘤中观察到AGO2的核丰富.
- 核AGO2去压缩的细胞质AGO2-eCLIP目标.
- 构成性核AGO2增强了细胞迁移.
- 德罗莎的耗尽限制了AGO2的细胞质,而TNRC6的耗尽促进了核AGO2的局部化.
结论:
- AGO2局部化是动态的,对环境线索,miRNA生物发生和miRISC辅因子表达有反应.
- 核AGO2可以导致基因标的去抑制和细胞行为的改变.
- 考虑AGO2局部化和miRISC机制对于理解miRNA角色至关重要.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Regulated mRNA Transport
6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
The Nucleolus
8.9K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.9K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Regulation of Expression Occurs at Multiple Steps
22.8K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.8K
Conservation of Protein Domains Over Different Proteins
10.9K
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.9K

