扩散泳促进了生物分子凝聚物的相位分离和运输
Viet Sang Doan1, Ibraheem Alshareedah2, Anurag Singh2
1Department of Mechanical and Aerospace Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260.
bioRxiv : the preprint server for biology
|July 18, 2023
概括
离子梯度驱动生物分子凝聚物的形成和运动. 这一过程被称为扩散性,增强了细胞内的生物分子运输和凝结寿命.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 化学热力学化学热力学
背景情况:
- 细胞微环境是异质的,包含各种有机体,如生物分子凝聚物.
- 生物分子凝聚物是细胞功能至关重要的没有膜的隔间.
- 细胞内的热力学梯度可以影响这些凝结物的行为.
研究的目的:
- 研究离子梯度对生物分子凝聚物运输的影响.
- 探索扩散泳在凝结物形成和动态中的作用.
- 了解非平衡力如何塑造细胞组织.
主要方法:
- 利用微流体平台创建受控的离子梯度.
- 在这些梯度下研究生物分子 (核酸,蛋白质) 的运输.
- 观察生物分子凝聚物的形成,运输和再分配.
主要成果:
- 离子度梯度通过扩散泳加速生物分子的运输.
- 局部生物分子丰富促进分相冷凝形成.
- 离子梯度会诱导活性运动,并增强凝结物的扩散.
- 梯度诱导的运动性通过动态再分配延长了凝结物的寿命.
结论:
- 扩散泳是一种关键的非平衡热力学力,控制了凝结物的行为.
- 离子梯度积极调节生物分子凝聚物的形成,运输和寿命.
- 这种机制提供了关于细胞组织和在异质环境中的功能的见解.
更多相关视频
08:02Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure
Published on: April 17, 2018
10.5K
07:57Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics
Published on: November 10, 2014
7.9K
相关概念视频
Analyte Adsorption and Distribution
700
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
700
Diffusion
193.8K
Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
193.8K
Facilitated Diffusion
510
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
510
Facilitated Transport
12.4K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
12.4K
Dialysis
734
Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
734
Centrifugation
2.3K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
2.3K
