分子核的性别确定? 两动物的实证证据
Chase H Smith1, Raquel Mejia-Trujillo1, Sophie Breton2
1Department of Integrative Biology, University of Texas at Austin, Austin, TX, USA.
bioRxiv : the preprint server for biology
|July 18, 2023
概括
在具有双重单亲遗传的淡水中,雄性线粒体DNA (mtDNA) 可能抑制雌性发育. 这项研究发现了线粒体小非编码RNA (sncRNAs) 沉默女性发育途径的证据.
科学领域:
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
- 线粒体基因组学 线粒体基因组学
背景情况:
- 核DNA通常决定动物的性别.
- 双重单亲遗传 (DUI) 的双胞胎可能涉及线粒体DNA (mtDNA) 在性别确定.
- 在DUI系统中,男性mtDNA (M mtDNA) 通过父亲遗传,类似于Y染色体,这表明它在男性的性别决定中发挥了作用.
研究的目的:
- 研究线粒体和核元素在淡水的性别确定途径中的作用.
- 为了验证mtDNA对DUI双体的性别确定有所贡献的假设.
- 探索线粒体小非编码RNAs (sncRNAs) 在性发育中的功能.
主要方法:
- 使用了DNA,RNA和线粒体sncRNA测序.
- 分析的重点是淡水 *Potamilus streckersoni* (Bivalvia: Unionida). 这种类型的鱼是主要的.
- 研究了基因表达和遗传差异化模式.
主要成果:
- 一个特定于男性的线粒体基因释放了一种针对女性发育和线粒体衰变途径的sncRNA.
- RNA测序证实,这种基因标在女性中是上调的,这表明线粒体sncRNA介导的基因沉默.
- 全基因组分析没有确定一个独特的核性别决定区域,尽管不能完全排除核因素.
结论:
- 线粒体DNA,特别是M mtDNA,似乎通过sncRNA机制抑制了DUI双体中的女性发育.
- 这项研究提供了线粒体位置的非呼吸功能和一种非常规的性别确定系统的证据.
- 需要进一步的研究,以充分阐明线粒体和核因素在双体性别决定中的相互作用.
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