相关实验视频

Updated: Jul 23, 2025

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

可回收的CRISPR/Cas9介导的基因破坏和删除在血清体中

Bastian Joehnk¹, Nebat Ali¹, Mark Voorhies¹

¹Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, USA.

bioRxiv : the preprint server for biology

|July 18, 2023

概括

克里斯普尔-Cas9技术能够有效地向菌类病原体质体中的基因干扰. 这一突破简化了基因功能的研究,克服了以前的基因操纵挑战.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

由于低同源重组频率,在二形真菌病原体histoplasma中的向基因破坏是困难的.
现有的方法导致DNA的异位整合或染色体外维护.
需要有效的遗传工具来研究质体病变的发生.

结论:

开发的CRISPR-Cas9系统显著提高了在质体中产生向突变的能力.
这种工具有助于对这一重要的真菌病原体中的基因进行功能分析.
能够更深入地了解质体生物学和病变发生.

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Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

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Published on: April 10, 2018

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

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