Jove
Visualize
联系我们

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.2K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.2K
Protein Folding01:22

Protein Folding

118.5K
Overview
118.5K
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Hierarchical multi-timescale structural dynamics of the disordered N-terminal of p53.

Nature communications·2026
Same author

Simple biological controllers drive the evolution of soft modes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

PEG-mCherry interactions beyond classical macromolecular crowding.

Protein science : a publication of the Protein Society·2025
Same author

The structural influence of the oncogenic driver mutation N642H in the STAT5B SH2 domain.

Protein science : a publication of the Protein Society·2024
Same author

The Conformational Space of the SARS-CoV-2 Main Protease Active Site Loops Is Determined by Ligand Binding and Interprotomer Allostery.

Biochemistry·2024
Same author

A speed limit on serial strain replacement from original antigenic sin.

Proceedings of the National Academy of Sciences of the United States of America·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 23, 2025

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.1K

在晶体中的功能性蛋白质动力学.

Eugene Klyshko1,2, Justin Sung-Ho Kim1,2, Lauren McGough3

  • 1Department of Physics, University of Toronto, Toronto, ON, Canada.

bioRxiv : the preprint server for biology
|July 18, 2023
PubMed
概括
此摘要是机器生成的。

了解蛋白质的运动是了解蛋白质功能的关键. 新的方法模拟了晶体中的蛋白质动态,有助于实验解释和可视化分子机器的作用.

更多相关视频

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.3K
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.4K

相关实验视频

Last Updated: Jul 23, 2025

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

2.1K
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

14.3K
Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip
10:45

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

8.4K

科学领域:

  • 生物物理学的生物物理.
  • 结构生物学 结构生物学
  • 计算生物学 计算生物学

背景情况:

  • 蛋白质通过运动来起作用,因此需要研究它们的动态.
  • 时间解析的X射线衍射提供了水晶中蛋白质运动的原子细节.
  • 实验的局限性需要补充计算方法来解释蛋白质动态.

研究的目的:

  • 开发和验证强大的分子动力学 (MD) 方法来模拟晶体内的蛋白质动力学.
  • 为了弥合实验观测和蛋白质运动的计算预测之间的差距.
  • 通过精确的模拟来增强对蛋白质功能机制的理解.

主要方法:

  • 建立了严格的协议来模拟晶体环境中的蛋白质动力学.
  • 专注于关键方面,如平衡,环境组成和力场选择.
  • 单个蛋白质链的总模拟时间超过7毫秒.

主要成果:

  • 确定了影响模拟和实验数据一致性的关键因素.
  • 证明模拟的蛋白质动力学准确地回顾了连接体诱导的构造变化.
  • 验证了开发的MD方法来捕获生物学相关的蛋白质运动.

结论:

  • 开发的模拟方法为水晶中的蛋白质动力学提供了准确和强大的洞察力.
  • 这项工作促进了计算模拟和实验技术之间的协同关系.
  • 能够增强对基本蛋白质功能运动的可视化和理解.