基于无氧微生物的导热性的气在线监测
Katharina Miebach1, Maurice Finger1, Alexandra Maria Katarina Scherer1
1Chair of Biochemical Engineering (AVT.BioVT), RWTH Aachen University, Aachen, Germany.
Biotechnology and bioengineering
|July 18, 2023
概括
本研究引入了一种使用热导电传感器的新型在线监测系统,用于在无氧微生物中有效分析 (H2) 生产. 这种方法加快了厌氧生物生产生物的表征.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 无氧微生物是可持续生物生产的关键.
- 为 (H2) 生产培养和表征无氧生物具有挑战性.
- 现有的H2传感器通常不适合无氧条件,因为需要氧气或湿度限制.
研究的目的:
- 开发一个并行,在线的H2监测系统,以有效地描述无气生物的H2产量.
- 评估热导电 (TC) 传感器对温度,湿度和CO2的交叉灵敏度.
- 为了验证系统的测量范围,使用已知的高和低H2产生的厌氧生物.
主要方法:
- 应用热导率 (TC) 传感器用于在线H2监测.
- 开发了一个并行系统,以节省时间进行表征.
- 评估了传感器对环境因素 (温度,湿度,CO2) 的交叉灵敏度.
- 通过Clostridium pasteurianum和Phocaeicola vulgatus验证了该系统.
主要成果:
- 在摇瓶种植中成功展示了在线H2生产监测.
- 通过使用集成的二氧化碳和压力测量,推导出H2转移速率和封闭的摩尔气体平衡.
- 计算无氧呼吸系数,以了解新陈代谢变化.
结论:
- 本文所介绍的在线H2监测方法加速了用于生物生产的无氧体表征.
- 该系统提供了介质成分和种植条件对H2产量的影响的见解.
- 这种方法可以在没有昂贵的设备或离线分析的情况下检测代谢变化.
关键词:
克洛斯特里迪亚米 (Clostridium pasteurianum) 是一种类植物.的 (Bacteroides vulgatus) 是一种常见的植物.厌氧微生物 厌氧微生物生物的生产生产.在线监测气转移率 在线监测气转移率热导电传感器 热导电传感器更多相关视频
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