相关实验视频

Updated: Jul 23, 2025

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

在改变诊断测试算法后,Clostridioides difficile感染的特征和结果

Andrew S Crone^1,2, Lorinda M Wright¹, Adam Cheknis¹

¹Research Service and Infectious Diseases Section, Edward Hines, Jr. VA Hospital, Hines, Illinois.

Infection control and hospital epidemiology

|July 18, 2023

概括

克洛斯特里迪奥伊德困难菌 (C. diff) PCR测试与毒素酶免疫测试 (EIAs) 结合,可以预测复发的C. diff感染. 一个两步测试策略可以提高C. diff复发的预测.

科学领域:

传染性疾病传染性疾病
临床微生物学临床微生物学
流行病学流行病学

背景情况:

聚合酶连锁反应 (PCR) 是一种用于检测Clostridioides difficile (C. diff.) 的敏感方法.
一些实验室使用两步算法,将PCR与毒素酶免疫试验 (EIA) 结合起来,以提高特异性.
了解PCR阳性/毒素阳性与PCR阳性/毒素阴性C. diff接触的结果至关重要.

研究的目的:

为了确定C. diffPCR阳性/毒素阳性接触的风险因素和结果.
为了将这些结果与C. diff的PCR阳性/毒素阴性遭遇进行比较.
评估C. diff复发的两步测试策略的预测价值.

主要方法:

在退伍军人事务医院进行了回顾性病例控制研究.
包括具有C. diffPCR阳性测试的患者,根据毒素EIA结果 (阳性或阴性) 分类.
分析了临床暴露,危险因素和30天后C. diff复发情况. 使用限制性内核酶分析 (REA) 进行了菌株类型化.

主要成果:

在130个C. diffPCR阳性遭遇中,38.5%是毒素EIA阳性,61.5%是毒素EIA阴性.
毒素阳性接触者接受治疗的频率更高 (96.0%与71.3%相比).
毒素阴性遭遇的30天复发风险较低 (OR,0.20). 较高的PCR周期值与较低的复发风险相关 (OR,0.82). 在毒素阴性病例中,REA组Y是常见的,而BI组在毒素阳性病例中占主导地位.

结论:

结合PCR和毒素EIA的测试策略可以帮助预测复发的C. diff感染.
这种两步方法提供了有价值的预后信息.
识别特定的C. diff菌株 (例如REA群) 可能进一步有助于预测结果.

更多相关视频

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

相关概念视频

Irritable Bowel Syndrome II: Clinical Features and Diagnostic Evaluation

Irritable Bowel Syndrome II: Clinical Features and Diagnostic Evaluation

Irritable Bowel Syndrome II: Clinical Features and Diagnostic Evaluation
Irritable Bowel Syndrome (IBS) is classified into subtypes based on the predominant bowel habits as determined by the Bristol Stool Form Scale (BSFS). The subtypes are:

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...

Appendicitis-II: Diagnostic Studies and Management

Appendicitis-II: Diagnostic Studies and Management

Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...