六种胺杀虫剂在瑞诺丁受体上的表征:耐药性和物种选择性
Jiahui Zhao1, Lianyun Lin1, Hadiatullah Hadiatullah1
1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency; Collaborative Innovation Center of Chemical Science and Engineering; School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Journal of agricultural and food chemistry
|July 18, 2023
概括
昆虫对二胺杀虫剂的耐药性是一个日益严重的问题. 这项研究揭示了兰利 (CYAN) 有效地向耐药昆虫的氨酸受体 (RyRs),提供了新的害虫控制策略.
科学领域:
- 分子生物学分子生物学
- 抗虫剂耐药性 抗虫剂耐药性 抗虫剂耐药性
- 药理学 药理学是指药理学的学科.
背景情况:
- 瑞诺丁受体 (RyRs) 是关键的杀虫剂点.
- 害虫RyRs中的耐药性突变限制了二胺杀虫剂的有效性.
- 耐药突变对杀虫剂活性的详细分子影响仍未得到充分研究.
研究的目的:
- 描述商业化胺杀虫剂对野生类型和耐药昆虫RyRs的活性.
- 调查抗虫剂耐药性和选择性的分子基础.
- 引导新型RyR向杀虫剂的开发.
主要方法:
- 已确立的HEK293细胞系表达野生类型和突变昆虫RyRs (Spodoptera frugiperda RyR).
- 利用一种基因编码的光蛋白 (R-CEPIA1er) 来监测内质网膜Ca2+水平.
- 在经过基因编辑的Drosophila中进行了时间延迟光测试和毒性测试.
主要成果:
- 西安特拉尼利普罗尔 (CYAN) 对所有经过测试的耐药昆虫RyRs.表现出高活性.
- 在基因编辑的Drosophila中,CYAN显示了最低的LD50,具有双重耐药RyRs.
- 与哺乳动物RyRs.相比,卜胺表现出最好的昆虫选择性.
结论:
- 胺杀虫剂的有效性受到RyR耐药性突变的显著影响.
- 是一种有前途的杀虫剂,用于控制耐药农业害虫.
- 了解RyR与杀虫剂的相互作用有助于开发有选择性和有效的害虫防治解决方案.
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