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生物性和化学性纳米颗粒对真核生物脂质膜模型的影响

Elena Piacenza¹, Kevin Sule², Alessandro Presentato¹

¹Department of Biological, Chemical and Pharmaceutical Science and Technologies, University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.

Langmuir : the ACS journal of surfaces and colloids

|July 18, 2023

概括

生物的纳米粒子 (bSeNPs) 固化了酵母细胞膜,与化学的SeNPs不同. 在bSeNP提取物中的有机物质显著影响其毒性和物理性质.

科学领域:

微生物纳米技术微生物纳米技术
生物性纳米材料合成
纳米粒子毒性机制的机制

背景情况:

微生物纳米技术生产生物金属和金属化物纳米材料 (NMs).
生物性NM正在研究用于抗微生物和抗癌应用.
生物源性NMS的毒性机制和影响需要进一步研究.

研究的目的:

为了评估生物纳米颗粒 (bSeNPs) 对Saccharomyces cerevisiae的毒性.
为了比较bSeNPs与化学性SeNPs (cSeNPs) 的毒性.
研究有机物质 (OM) 对bSeNP毒性和物理化学性质的影响.

主要方法:

使用Stenotrophomonas maltophilia菌株SeITE02.02生产bSeNP的方法
使用富里埃变换红外光谱学对bSeNP及其有机物质 (OM) 的表征.
对纳米粒子与模仿真核细胞膜的脂质囊泡相互作用的评估.

主要成果:

生物纳米颗粒提取物介于脂质囊泡的整体刚性化.
化学SeNPs对脂质囊泡流动性表现出微不足道的影响.
有机物质 (OM) 和cSeNPs_OM对bSeNP提取物产生了类似的修改,突出了OM的作用.

结论:

与bSeNPs相关的有机物质显著影响它们与细胞膜的相互作用.
了解OM的作用对于评估生物性纳米材料的真正生物特性至关重要.
需要进一步的研究来阐明生物性纳米材料,它们的相关有机物质和细胞毒性之间的复杂相互作用.

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