模仿肌微环境以增强骨肌肉粘附和长寿在一个功能性的微型杆平台
Himanshi Jangir1, James J Hickman1
1Nanoscience Technology Center, University of Central Florida, 12424 Research Pkwy, Orlando, Florida 32826, United States.
ACS biomaterials science & engineering
|July 18, 2023
概括
研究人员开发了一种模仿自然肌的新型微环境,以改善骨肌肉细胞在微型肌肉上的附着. 这项创新使28天的稳定,机械活性肌管培养能够用于慢性药物疗效和毒性研究.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 骨肌肉生理学 骨肌肉生理学
背景情况:
- 微型杆平台用于体外骨肌肉力量动力学.
- 肌管脱离悬臂限制了药物测试的长期研究.
- 现有的方法阻碍了对肌肉功能的慢性研究.
研究的目的:
- 为长期实验增强肌管与微型输送器的集成.
- 模仿自然肌微环境,以改善细胞粘附.
- 为了使骨肌药物疗效和毒性的慢性调查.
主要方法:
- 在西兰基板上模仿肌微环境,使用弹性素,原蛋白,肝素硫酸蛋白质糖和氨酸.
- 培养的初级和诱导多能干细胞 (iPSC) 衍生的人类骨肌肉髓管.
- 集成的神经管与玻璃和微型杆系统.
主要成果:
- 实现了机械活性肌管的长期存活超过28天.
- 证明了稳定的肌管培养长达五周.
- 在初级和iPSC衍生骨肌中观察到类似的疲劳指数趋势.
- 在一个月的时间里,成功地在微型杆系统上测试了肌管.
结论:
- 生物模拟肌微环境显著改善肌管粘附和微型杆的生存.
- 这种方法使得骨肌功能的慢性体外研究成为可能.
- 使用先进的芯片上的肌肉模型,促进长期药物疗效和毒性评估.
相关概念视频
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Bone, or osseous tissue, is a connective tissue that has a large amount of two different types of matrix material. The organic matrix is similar to the matrix material found in other connective tissues, including some amount of collagen and elastic fibers. This gives strength and flexibility to the tissue. The inorganic matrix consists of mineral salts— mostly calcium salts— that give the...