Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Simple Clinical Prediction Model for Metabolic Dysfunction-Associated Steatotic Liver Disease in Adolescents with Obesity.

Indian journal of pediatrics·2026
Same author

Pregnancy-induced tissue-resident memory-like T cells contribute to tumor control in breast cancer.

Nature immunology·2026
Same author

Primary duct closure after laparoscopic common bile duct exploration in elderly patients with cholecystocholedocholithiasis: a propensity-matched analysis.

BMC surgery·2026
Same author

Evidence supporting the role of GIGYF2 in synapse development and autism.

Molecular psychiatry·2026
Same author

A Phase 1 and Biodistribution study of Ifabotuzumab, a humanized agonistic EphA3-targeted antibody, in patients with recurrent glioblastoma.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

MYLIP-dependent ubiquitination and degradation of LDLR in acute myeloid leukemia and MAPK signaling.

Molecular and cellular probes·2026

相关实验视频

Updated: Jul 23, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K

cellCounts:一个R函数,用于量化10倍单细胞RNA测序数据.

Yang Liao1,2, Dinesh Raghu1,2, Bhupinder Pal1,2

  • 1Olivia Newton-John Cancer Research Institute, Heidelberg, Victoria 3084, Australia.

Bioinformatics (Oxford, England)
|July 18, 2023
PubMed
概括

cellCounts是一个新的R工具,用于从10x Genomics Chromium单细胞RNA测序数据中准确量化基因表达. 它有效地处理庞大的数据集,性能优于现有的工具,如cellRanger和STARsolo.

更多相关视频

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
04:21

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

2.9K
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.2K

相关实验视频

Last Updated: Jul 23, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.6K
Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy
04:21

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

2.9K
Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.2K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 10x Genomics Chromium技术能够实现高通量单细胞RNA测序,产生大量的数据.
  • 从庞大的单细胞RNA测序数据集中量化基因表达提出了重大的计算挑战.

研究的目的:

  • 介绍 cellCounts,一种基于 R 的新型工具,用于从 10x Genomics Chromium 数据中高效准确量化基因表达.
  • 为了应对单细胞RNA测序分析中大量数据所带来的挑战.

主要方法:

  • cellCounts使用种子和投票策略来与参考基因组进行读取对齐.
  • 它读取独特的分子标识符 (UMI),并使用featureCounts程序将UMI分配给基因.
  • 该工具在R包Rsubread中实现,以便与其他基于R的生物信息工作流程无集成.

主要成果:

  • 使用模拟和真实数据集的评估表明,cellCounts与已建立的工具 (如cellRanger和STARsolo) 相比,提供了有利的性能.
  • cellCounts为单细胞水平的基因表达量化提供了一种高效准确的解决方案.

结论:

  • cellCounts为研究人员提供了一种有价值和可访问的工具,用于使用10x Genomics单细胞RNA测序数据.
  • 其高效的处理和准确的量化能力,为细胞基因表达特征提供了更深入的见解.