状态过渡的依赖性在阿拉比多和大麦植物的照明时间
Daria V Vetoshkina1, Marina A Kozuleva2, Ivan I Proskuryakov2
1Institute of Basic Biological Problems of the Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Russia. vetoshkinadv@gmail.com.
Protoplasma
|July 18, 2023
概括
植物中的状态转换 (ST) 涉及光系统之间的光收集复合II (LHCII) 运动. 阿拉比多普西斯的ST发育速度比大麦慢,这与甲状腺膜粘度有关,而不是蛋白质酸化.
科学领域:
- 植物生理学 植物生理学
- 光合作用研究研究光合作用.
- 分子植物科学 分子植物科学
背景情况:
- 状态转换 (ST) 调节植物的光系统II (PSII) 和光系统I (PSI) 之间的光能分布.
- ST涉及与PSII (L-LHCII) 相关的光采集复合体II (LHCII) 的可逆迁移到PSI.
研究的目的:
- 调查Arabidopsis thaliana (双胞胎) 和Hordeum vulgare (单胞胎) 之间状态过渡的动力学上的差异.
- 阐明导致ST发育观察到的动力变异的潜在分子和生物物理机制.
主要方法:
- 在77K和室温下进行叶绿素a光测量,以评估ST动力学.
- 对Lhcb1和Lhcb2蛋白质酸化水平的分析.
- 电子偏磁共振 (EPR) 用于确定甲状腺膜粘度.
- 焦显微镜用于比较叶绿体大小.
主要成果:
- 与大麦叶相比,Arabidopsis叶中的状态1到状态2的过渡发生的速度明显慢.
- 两种物种之间没有观察到Lhcb1和Lhcb2蛋白质的酸化率的差异.
- 在阿拉比多普西斯中,thylakoid膜的粘度高于大麦中的粘度.
- 阿拉比多普西斯的 хлоропласт比大麦中的更大.
结论:
- 在Arabidopsis中,状态转换的发展速度较慢,这归因于甲状腺膜内L-LHCII复合体的迁移速度减少.
- 阿拉比多普西斯的较高甲状腺膜粘度可能会阻碍L-LHCII复合体的移动性,从而减缓状态过渡.
- 叶绿体大小的差异也可能导致二叶植物和单叶植物之间ST效率的差异.
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