通过高分辨率移动性分析实时研究不同狭义的形状的罗塔病毒双层粒子之间的自发互转
Juan Fernandez de la Mora1, Fernando Almazán2, Javier M Rodríguez3
1Department of Mechanical Engineering and Materials Science, Yale University, 9 Hillhouse Avenue, New Haven, Connecticut 06520-8286, United States.
Analytical chemistry
|July 18, 2023
概括
罗塔病毒双层颗粒 (DLP) 在气相中表现出多种构造,揭示了它们的结构和潜在不稳定性的洞察力. 这些发现表明溶液中已经存在结构变异,可能是由于VP6蛋白质损失造成的.
科学领域:
- 结构生物学 结构生物学
- 病毒学 病毒学
- 物理化学 物理化学
背景情况:
- 罗塔病毒双层颗粒 (DLP) 是关键的病毒结构.
- 了解DLP构造是病毒组合和稳定性的关键.
- 之前的研究暗示了结构变化,但缺乏实时气相分析.
研究的目的:
- 为了研究轮状病毒DLP的气相形状.
- 分析DLP结构互换的动态和可逆性.
- 为了将气相结构与潜在的溶液相形状和VP6蛋白质损失相关联.
主要方法:
- 气相中的DLP的高分辨率差分移动性分析 (DMA).
- 从水性酸溶液中进行电喷气电离.
- 实时监测粒子构造和相互转换.
主要成果:
- 在气相中,多达七种不同的DLP形状得到了解决.
- 观察到这些形状之间的快速,可逆的相互转换.
- 与溶液 (70 nm) 相比,气相DLP的直径较小 (60 nm),这表明结构变化和潜在的VP6三元组五重奏损失.
结论:
- 观察到的气相形状可能源于溶液中先前存在的形状.
- 不完整的DLP,可能缺少VP6的三角五重奏,可能解释了观察到的结构多样性.
- 在酸中对多种轮状病毒类型的VP6损失的系统观察表明,这可能是电解质特异性的特征.
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