纳米喷雾消毒电喷雾电离 (Nano-DESI) 质谱成像与高离子移动性分辨率成像
Li-Xue Jiang1, Emerson Hernly1, Hang Hu1
1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.
Journal of the American Society for Mass Spectrometry
|July 18, 2023
概括
这项研究引入了一种新的纳米喷雾脱吸电喷雾电离 (纳米-DESI) 平台,并与被困离子移动性光谱 (TIMS) 配合用于质谱成像 (MSI). 增强的平台有效地分离组织中的脂质异构体和异构体,改善MSI实验中的分子特异性.
科学领域:
- 分析化学 分析化学
- 生物物理学的生物物理.
- 生物化学 生物化学
背景情况:
- 在质谱成像 (MSI) 中,异构体和异构体物种的非有针对性的分离是一个重大挑战.
- 离子移动光谱 (IMS) 与MSI相结合,通过区分这些物种来增强分子覆盖和特异性.
研究的目的:
- 实施和评估一个纳米喷雾脱电喷雾电离 (纳米-DESI) 源与捕获的离子移动性光谱 (TIMS) 的MSI.
- 评估纳米-DESI-TIMS-MSI平台在分离和定位生物组织中脂质异构体和异构体中的能力.
主要方法:
- 一个新的纳米-DESI源和入口扩展被构建用于与TIMS质谱仪集成.
- 纳米-DESI-TIMS-MSI系统被用于成像小鼠大脑组织部分.
- 使用狭窄的移动性扫描窗口实现了高离子移动性分辨率,专注于脂质分子.
主要成果:
- 纳米-DESI-TIMS-MSI平台展示了高离子移动性分辨率 (高达300),超过了之前的漂流管IMS研究.
- 成功地分离了脂质异构体和异构体,揭示了它们在组织中的独特空间分布.
- 该平台增强了MSI分析的特异性和分子覆盖范围.
结论:
- 高移动分辨率IMS,特别是开发的纳米-DESI-TIMS-MSI平台,是分析复杂生物分子混合物的强大工具.
- 这种方法显著提高了在MSI中区分和定位异构体和异构体物种的能力.
- 这些发现强调了先进IMS-MSI技术的潜力,用于详细的生物组织分析.
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