三胞胎双胞胎诺芬在 orofacial 疼痛并发症中的女性特异性作用
Hui Shu1, Sufang Liu1, Joshua Crawford1
1Department of Biomedical Sciences, Texas A&M University School of Dentistry, Dallas, TX, United States.
Pain
|July 18, 2023
概括
这项研究揭示了三神经中的迪诺芬在关节疾病 (TMD) 和偏头痛之间的联系中起着关键作用,特别是在女性中. 这一发现为共患性疼痛疾病提供了新的见解.
科学领域:
- 神经科学是一个神经科学.
- 疼痛研究 疼痛研究
- 遗传学 是一个遗传学.
背景情况:
- 偏头痛和部疾病 (TMDs) 经常一起发生,但潜在的机制仍然不清楚.
- 研究TMD和偏头痛的并发症对于理解复杂的疼痛状况至关重要.
研究的目的:
- 使用小鼠模型探索肌源性TMD和偏头痛之间的联系机制.
- 确定神经化学物质在TMD和偏头痛的并发症中的性别特异性作用.
主要方法:
- 一个小鼠模型是通过通过masseter肌肉肌绑定 (MMTL) 诱导TMD来创建的.
- 酸甘油 (NTG) 用于诱导与先前存在的TMD的小鼠的偏头痛类似的疼痛.
- 用RNA测序和化学遗传学来研究前诺芬 (Pdyn) 在脊柱三叉尾核 (Sp5C) 中的作用.
主要成果:
- MMTL加NTG在雌性小鼠的Sp5C中增加了Pdyn mRNA表达,但不是雄性小鼠.
- 抑制Pdyn表达神经元或在Sp5C中使用抗丁诺芬抗血清可以缓解女性的TMD诱发过敏和偏头痛般的疼痛.
- 激活Pdyn表达神经元或在Sp5C中注射dynorphin A在女性中诱导了类似偏头痛的疼痛,取决于NTG.
结论:
- 三胞胎诺芬在调节TMD和偏头痛的并发症方面发挥着显著的,女性特异性的作用.
- 这些发现突出了共发性TMD和偏头痛的潜在治疗点,特别是在女性中.
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