结合新陈代谢工程和脂质液滴储存工程,以改善Yarrowia Lipolytica中的α-Bisabolene生产
Zhihui Lu1, Yaping Wang1, Zhuo Li1
1State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, No.29 the 13th Street TEDA, Tianjin 300457, PR China.
Journal of agricultural and food chemistry
|July 18, 2023
概括
研究人员在Yarrowia lipolytica中增强了微生物的α-bisabolene生产,这是一个有价值的基烯. 这种新的策略使用废油实现了创纪录的1954.3mg/L标位,为可持续的生物制造铺平了道路.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 阿尔法-比萨博是一种多用途的基烯,在各种行业都有应用.
- 微生物生产为传统方法提供了可持续和高效的替代方案.
- 雅罗维亚 (Yarrowia lipolytica) 是一个强大的宿主,可以生产有价值的化合物.
研究的目的:
- 在Yarrowia lipolytica中增强alpha-bisabolene的生产.
- 为了优化阿尔法-比萨博生物合成途径的代谢流量.
- 通过将其隔离在脂质滴中来减轻α-bisabolene的毒性.
主要方法:
- 通过过度表达DGA1和OLE1基因来改造Yarrowia lipolytica.
- 用辅助碳来源补充培养.
- 利用废弃的油作为发酵的碳来源.
主要成果:
- 取得了1954.3毫克/升的阿尔法-比萨博标位纪录.
- 与对照菌株相比,表现出96倍的增加.
- 证实了增加脂质含量和不和脂肪酸的重要作用.
结论:
- 新的策略显著改善了Yarrowia lipolytica中的α-bisabolene生物生产.
- 废食用油是高铁生产的可行基质.
- 开发的方法为生产高价值烯提供了一个可扩展的平台.
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