相关实验视频
Updated: Jul 23, 2025
07:47
Microscopy of Fission Yeast Sexual Lifecycle
Published on: March 9, 2016
14.6K
裂变酵母细胞大小控制系统集成了测量细胞表面积,体积和时间的途径
Kristi E Miller1, Cesar Vargas-Garcia2, Abhyudai Singh3
1Department of Biochemistry and Cell Biology, The Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
Current biology : CB
|July 18, 2023
概括
裂变酵母细胞分裂由细胞表面积 (SA) 控制在一个值下,转向大尺寸的基于体积的控制. 化会影响这个值,集成的途径报告SA,体积和精确细胞大小调节的时间.
科学领域:
- 细胞生物学 细胞生物学
- 分子和发育生物学分子和发育生物学
背景情况:
- 细胞表现出精确的尺寸控制,但控制分裂的特定尺寸参数 (例如表面积,体积) 仍然在很大程度上未被阐明.
- 裂变酵母分裂部分受蛋白激酶Cdr2的调节,该蛋白激酶与细胞表面积 (SA) 相关.
研究的目的:
- 研究分裂酵母细胞大小控制的机制.
- 要确定细胞分裂主要是由细胞表面积或体积调节的.
- 阐明特定蛋白质 (Cdr2,Cdc25,Cdc13) 在整合细胞分裂的大小和时间线索中的作用.
主要方法:
- 在野生型和突变裂变酵母菌株中细胞分裂的比较分析.
- 在细胞周期期间研究蛋白质积累动态 (Cdc25,Cdc13).
- 数学建模和实验验证以整合信号通路.
主要成果:
- 分裂酵母细胞仅在特定尺寸值以下以表面积 (SA) 进行分裂.
- 导致更大的细胞大小的突变诱导了对基于体积的分裂控制的转变.
- 二倍体细胞的分裂大于二倍体细胞,但保留了基于SA的分裂,这表明二倍体细胞为切换控制机制设置了门.
- Cdc25积累与体积相关,而Cdc13核积累则充当定时器,暗示集成信号.
结论:
- 分裂酵母中的细胞大小控制是一个复杂的系统,集成了多个输入:细胞表面积 (Cdr2),细胞体积 (Cdc25) 和细胞周期时间 (Cdc13).
- 这种集成系统允许灵活的细胞大小控制,根据生长条件和 ploidy,表现出增大和增加的属性.
- 排列性是尺寸值的一个关键决定因素,在这个值下,划分控制从表面积转移到体积.
相关概念视频
02:36Cells Coordinate Growth and Proliferation
4.5K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.5K
01:28Yeast Signaling
14.7K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.7K
01:22Cell Size
115.0K
Cell sizes vary widely among and within organisms. Bacterial cells range between 1-10 micrometers (μm)and are considerably smaller than most eukaryotic cells. The smallest bacteria are 0.1 μm in diameter—about a thousand times smaller than eukaryotic cells, which typically range from 10-100 μm.
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...
115.0K