对比蛋白质组学揭示了Cryptosporidium parvum感染破坏了细胞屏障
Luyang Wang1, Zhaohui Cui2, Na Li1
1College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450046, China.; Key Laboratory of Quality and Safety Control of Poultry Products (Zhengzhou), Ministry of Agriculture and Rural Affairs, PR China.
Journal of proteomics
|July 18, 2023
概括
杆菌感染会破坏宿主细胞膜和细胞骨,影响屏障功能. 这项研究确定了参与寄生虫粘附和入侵的关键宿主蛋白质,促进了对密码菌病原体的理解.
科学领域:
- 寄生虫学的寄生虫学
- 细胞生物学 细胞生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 杆菌是一种重要的原生虫寄生虫,导致腹疾病和死亡率.
- 对C. parvum感染和病原体的分子机制尚不清楚.
研究的目的:
- 为了研究宿主细胞膜蛋白在C. parvum感染期间的变化.
- 识别潜在的宿主细胞受体,了解C. parvum和宿主细胞之间的分子相互作用.
主要方法:
- 使用定量双重质量标签 (TMT) 液体染色学-双重质谱学对感染C. parvum的HCT-8细胞进行蛋白质组学分析.
- 通过定量实时逆转录聚合酶连锁反应和西部抹杀,提取原生膜蛋白和验证差异表达的蛋白质.
主要成果:
- 在感染后6小时确定了4844种可量化的蛋白质,其中625种上调和116种下调 (1.5倍变化,p < 0.05).
- 不同表达的蛋白质主要与细胞骨和细胞质矩阵功能有关.
- 帕菌感染似乎会破坏细胞间粘附和宿主细胞屏障功能.
结论:
- 宿主细胞膜蛋白对于C. parvum的粘附和入侵至关重要.
- C. parvum感染显著改变宿主细胞骨蛋白质,并损害细胞屏障的完整性.
- 这项研究提供了洞察力,对宿主-寄生虫相互作用的分子机制,在cryptosporidiosis.
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