来自Streptomyces sp.的基于脂肪酸的抗微生物. SORS-24是一种从Sonchus oleraceus中分离出来的内生植物
Rabia Tanvir1, Imran Sajid2, Yasir Rehman3
1Institute of Microbiology (IOM), University of Veterinary and Animal Sciences (UVAS), Lahore, Punjab 54000, Pakistan.
Letters in applied microbiology
|July 18, 2023
概括
一种新型的Streptomyces tanashiensis菌株产生脂肪酸抗微生物,胺和胺,对大肠杆菌和B. subtilis.等细菌表现出强烈的活性. 这一发现为抗微生物抗药性增加带来了新的希望.
科学领域:
- 微生物学和天然产品化学
- 抗微生物耐药性研究研究
背景情况:
- 增加细菌耐药性需要使用替代抗微生物策略.
- 基于脂肪酸的抗微生物药物提供非特异性的作用模式,使它们成为有希望的候选药物.
研究的目的:
- 为了研究从Sonchus oleraceus中分离出来的新型细菌菌株的抗菌潜力.
- 为了识别和描述该菌株产生的抗微生物化合物.
- 评估分离物及其化合物的基因毒性和抗氧化活性.
主要方法:
- 来自Sonchus oleraceus的细菌菌株SORS 24 (Streptomyces tanashiensis) 的分离和鉴定.
- 对各种细菌和藻类菌株进行抗菌敏感性测试.
- 高性能液体染色学-质谱学 (HPLC-MS) 用于化合物识别.
- 脂肪酸胺基 (胺基和胺基) 的净化和表征.
- 使用缺乏PolA的大肠杆菌菌株进行基因毒性测定.
- 烯烯水 (DPPH) 抗氧化剂测定.
- 16S rRNA基因测序用于精确的物种识别.
主要成果:
- 菌株SORS 24对大肠杆菌,大肠杆菌,细菌菌和 Pseudomonas sp. 呈现出显著的抗菌活性.
- 该菌株表现出抗氧化活性 (EC50 = 0.8 μg/ml) 和对盐水的中度毒性 (LC50 = 10 μg/ml).
- 通过HPLC-MS分析确定了oleamide和erucamide作为活性化合物,两者都对B. subtilis和E. coli表现出强烈的活性.
- 这种菌株及其产生的胺对大肠杆菌K-12 AB 3027.27具有显著的基因毒性.
- 16S基因测序证实了该菌株是Streptomyces tanashiensis.
结论:
- 菌株Streptomyces tanashiensis SORS 24产生胺和胺,这是基于脂肪酸的新型抗菌剂.
- 这些化合物具有显著的抗菌和基因毒性潜力,需要进一步研究治疗应用.
- 这项研究代表了Streptomyces tanashiensis生产这些特定脂肪酸抗菌素的首份报告.
相关概念视频
Bacterial Phylum Actinobacteria
36
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
36
Biosynthesis of Lipids
40
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
40
Antimicrobial Proteins
1.0K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
1.0K
Surface Membrane Barriers
1.2K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.2K
Bacterial Phylum Firmicutes
47
Firmicutes is a diverse phylum of Gram-positive bacteria characterized by a low GC content in their genomes. This phylum includes organisms with monoderm or diderm cell envelopes, highlighting a complex evolutionary history. Firmicutes comprises several major orders, including Lactobacillales, Clostridiales, and Bacillales, which exhibit remarkable diversity in their morphology, metabolism, and ecological roles.The order Lactobacillales includes lactic acid bacteria, which are fermentative...
47
Microorganisms in Agriculture and Food industry
88
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
88


