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用CRISPR驱动的基因组工程用于 Chorismate 和 Anthranilate 积累的Corynebacterium 细胞工厂

Hye-Jin Kim¹, Si-Sun Choi¹, Eung-Soo Kim¹

¹Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Republic of Korea.

Journal of microbiology and biotechnology

|July 18, 2023

概括

这项研究设计了*Corynebacterium glutamicum*以使用CRISPR基因组编辑来提高chorismate和antranilate的生产. 修改后的菌株取得了这些关键的基胺路径中间体的显著产量.

科学领域:

代谢工程是代谢工程.
合成生物学合成生物学
微生物生物技术微生物生物技术

背景情况:

石基胺通路对于生产芳香化合物至关重要.
提高 chorismate (CHR) 和 anthranilate (ANT) 的积累对于工业应用至关重要.
再组合*Corynebacterium glutamicum*菌株为微生物生产提供了潜力,但需要优化.

研究的目的:

为了改造 *Corynebacterium glutamicum* 以提高 chorismate 和 anthranilate 的产量.
利用CRISPR驱动的基因组工程进行精确的修改.
建立一个强大的平台,用于生产神基酸途径代谢产物.

主要方法:

基于CRISPR的基因组工程来修改什基马特通路.
反耐药酶和路径组件的过度表达 (例如AroG,AroH,AroF,AroB,QsuC).
代谢途径的优化,包括碳代谢和竞争途径的删除 (例如,IolR,Ack,Pta).

主要成果:

成功生成了*C. glutamicum*细胞工厂,增强了CHR和ANT的生产.
在微型培养系统中, Chorismate 的产量为 0.48 g/l,Anthranilate 的产量为 0.9 g/l.
证明了合理的细胞工厂设计策略的有效性.

结论:

在CRISPR驱动的基因组重新设计是有效的增强shikimate途径代谢物生产.
开发的*C. glutamicum*平台适用于高产的芳香化合物菌株.
这一策略为生产来自CHR和ANT的有价值化学品提供了基础.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?科里尼细菌 (Corynebacterium) 是一种细菌人类酸盐是什么?合唱团成员合唱团成员基因组编辑基因组编辑

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