从大麦和杂草中的生产由超热友细菌,Cadicellulosirupter bescii
Minseok Cha1, Jun-Ha Kim2, Hyo-Jin Choi2
1Research Center for Biological Cybernetics, Chonnam National University, Gwangju 61186, Republic of Korea.
Journal of microbiology and biotechnology
|July 18, 2023
概括
工程细菌显著提高了从植物废物 (如大麦和miscanthus) 中产生生物的产量. 这一进步提供了一种更有效的方法,用于从基纤维素生物质中获得可持续能源和生物化学品.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 可再生能源可再生能源是可再生能源.
- 微生物生理学 微生物生理学
背景情况:
- 富含纤维素,半纤维素和素的植物生物质对分解具有反抗性,这对生物燃料生产构成了挑战.
- 超热友细菌*Caldicellulosiruptor bescii*可以有效地利用纤维素原料来获取能量.
- "C. bescii"的代谢工程为增强和其他有价值的生物化学物质的生产提供了潜力.
研究的目的:
- 评估使用*Caldicellulosiruptor bescii*从大麦草和miscanthus生产生物的可行性.
- 为了比较野生型菌株 (JWCB001) 和*C. bescii*的工程菌株 (JWCB018) 的和酸盐生产能力.
主要方法:
- 培育了两种*C. bescii*菌株:JWCB001 (野生型) 和JWCB018 (工程,ΔpyrFA Δldh ΔcbeI*).
- 化纤维素原料的发酵:大麦和杂草.
- 对两种菌株的和酸盐生产量化.
主要成果:
- 与野生型JWCB001.018相比,改造的JWCB018菌株表现出明显更高的酸盐和的产量.
- JWCB018从大麦草中产生了大约54%的酸盐和63%的气.
- 从miscanthus,JWCB018相对于JWCB001产生了25%更多的和33%更多的酸盐,这表明成功地重定向了代谢途径.
结论:
- 工程*C. bescii*,特别是菌株JWCB018,在增强生物和酸生产的高效.
- 这项研究支持工程 *C. bescii* 的潜力,作为从各种植物生物质来源可持续生产和其他生物化学品的强大平台.
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