乙酸盐以氧化应激依赖的方式减轻纤维化
Chiaki Kawabata1, Yosuke Hirakawa1, Reiko Inagi2
1Division of Nephrology and Endocrinology, The University of Tokyo Graduate School of Medicine, Tokyo, Japan.
Physiological reports
|July 18, 2023
概括
乙酸盐是一种常见的肠道细菌副产品,通过减少氧化应激和改善线粒体功能来保护脏免受损伤. 这项研究表明乙酸作为慢性病 (CKD) 的治疗剂的潜力.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 短链脂肪酸 (SCFA),是由食纤维的肠道微生物发酵产生的,积极影响宿主生理.
- 乙酸盐是人类中最丰富的SCFA,在急性损伤模型中表现有前途.
- 特别是在慢性脏病 (CKD) 中,SCFA 的脏保护机制需要进一步研究.
研究的目的:
- 调查乙酸和其他SCFA对脏损伤的脏保护作用.
- 阐明乙酸在减轻细胞中的氧化应激和线粒体功能障碍中的作用,以及CKD的体内模型.
主要方法:
- 在体外研究中,使用暴露于氧化应激的人类2 (HK-2) 细胞.
- 线粒体分析,包括MitoSOX染色和线粒体动态的基因表达.
- 在体内实验中,使用单边尿路阻塞 (UUO) 的小鼠模型来诱导纤维化.
主要成果:
- 乙酸盐治疗增强了HK-2细胞活力,并在氧化应激下减少了反应性氧物种 (ROS) 的产生.
- 乙酸降低了线粒体ROS,并在体外调节了线粒体分裂相关基因的表达.
- 在体内,乙酸盐改善了UUO诱导的纤维化,减少了氧化应激标志物γH2AX,并改善了近道管中的线粒体形态.
结论:
- 乙酸在损伤的体外和体外模型中都显示出显著的保护作用.
- 乙酸通过减少氧化应激和改善线粒体功能来减轻损伤.
- 这些发现表明,乙酸对治疗慢性病 (CKD) 具有治疗潜力.
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