中链脂肪酸以PPAR β/δ-依赖的方式改变代谢和炎症基因的巨细胞表达
Paula V Gaete1, Luz D Nieves-Barreto2, Valentina Guatibonza-García2
1Department of Physiological Sciences, Universidad Nacional de Colombia, Bogotá, Colombia.
Scientific reports
|July 18, 2023
概括
中链脂肪酸 (MCFA) 通过增强胆固醇外流和减少巨细胞的炎症来促进心血管健康,部分是通过过氧酶增殖器激活受体β/delta (PPAR β/δ) 信号传递.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 心血管健康 心血管健康
背景情况:
- 中链脂肪酸 (MCFA) 对心血管健康具有重要意义.
- 了解它们在巨细胞中的分子机制至关重要.
研究的目的:
- 为了研究MCFA对脂质代谢和巨细胞炎症基因表达的影响.
- 确定氧酶增殖器激活受体β/delta (PPAR β/δ) 在调解这些效应中的作用.
主要方法:
- 鼠类巨细胞 (J774A.1) 用MCFA (八酸,十酸),长链脂肪酸 (棕酸) 或PPARβ/δ激动剂进行治疗.
- 实验涉及脂聚糖 (LPS) 刺激和PPARβ/δ使用siRNA. knockdown的试验.
- 分析了基因表达,细胞脂质吸收和蛋白质表达.
主要成果:
- 部分依赖PPAR β/δ的方式,MCFA增加了Plin2 (抗炎蛋白) 和ABCA1 (胆固醇排泄) 的表达.
- 酸还刺激了Pltp,帮助细胞脂质排放.
- 亲炎性基因 (Il6,Tnf,No2,Mmp9) 是由棕酸盐上调的,而不是MCFA.
- 在巨细胞中,MCFA降低了脂蛋白内部化.
结论:
- MCFA调节巨细胞的转录反应,有利于胆固醇排放,减少炎症.
- 这些有益效应部分由PPAR β/δ介导.
- 与palmitate等长链脂肪酸相比,MCFA在炎症基因表达方面表现出更有利的特征.
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