通过小ORF编码的多调节乙酸盐耐受性的调节,调节Methylomonas sp.中的排泄特异性. 在 DH-1 DH-1 中
Seungwoo Cha1, Yong-Joon Cho2, Jong Kwan Lee1
1School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
Biotechnology for biofuels and bioproducts
|July 18, 2023
概括
WatR调节细胞对甲类动物中弱有机酸的防御. 它激活了WatABO排泄和小聚,增强耐受性,特别是对乙酸盐,以改善生物处理.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 代谢工程是代谢工程.
背景情况:
- 甲类植物是将甲转化为有价值的化学物质的关键.
- 了解有机酸耐受性对于优化生产至关重要.
- WatR是一种LysR类调节剂,最初与Methylomonas sp.的乳酸耐受性有关. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH.
研究的目的:
- 研究WatR在抗弱有机酸的细胞防御中的作用.
- 确定由WatR调节的新型基因.
- 阐明甲类动物中酸性耐受性的机制.
主要方法:
- 对WatR.的全基因组结合分析.
- 转录调节研究.
- 基因删除和过度表达的实验.
主要成果:
- 确定了RND型排水 (WatABO) 和小型开放式读取框架 (smORF,watS1-watS5) 作为WatR目标.
- 瓦塔博调解了对各种弱有机酸 (甲酸,乙酸,乳酸,酸) 的耐受性.
- 在乙压力下,WatR激活WatABO和watS基因;WatS1增强了的乙特异性.
结论:
- WatR是Methylomonas sp.中细胞防御弱有机酸,特别是酸的关键转录因子. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH-1. DH.
- WatABO 排泄和 WatS 聚被确定为 WatR 监管的酸耐受性标.
- 这些发现有助于更好地了解甲类动物对弱酸耐受性的理解,有助于开发用于甲转换的生物工艺.
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