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用Corynebacterium glutamicum的代谢工程来从葡萄糖和小麦水解盐中生产脂肪酒精

Felix Werner¹, Lynn S Schwardmann², Daniel Siebert^1,3

¹Microbial Biotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstraße 53, 94315, Straubing, Germany.

Biotechnology for biofuels and bioproducts

|July 18, 2023

概括

这项研究利用Corynebacterium glutamicum从第二代原料中生产脂肪酒精 (FAL). 开发的微生物平台可以从小麦水解液中有效地进行FAL生物合成,为石化生产提供可持续的替代品.

科学领域:

代谢工程是代谢工程.
合成生物学合成生物学
生物技术是生物技术.

背景情况:

脂肪醇 (FAL) 是一种有价值的化学物质,在化品,滑剂和生物燃料中越来越多的应用.
目前的FAL生产依赖于石化或第一代生物基原料,这引发了环境和生物多样性问题.
从第二代原料中微生物生产FAL是一种可取的可持续替代品.

研究的目的:

从第二代原料中设计Corynebacterium glutamicum以有效地生产脂肪酒精 (FAL).
为可持续的FAL生物合成开发一个微生物平台,减少对化石资源和第一代原料的依赖.
为了建立代谢工程的原则,以生产工业化学品从纤维素生物质.

主要方法:

通过删除fasR和过度表达脂肪乙基-CoA减少酶 (FAR) 基因来放松脂肪酸生物合成的调节.
减弱的原生铁酶表达和集成的树脂利用途径.
采用适应性实验室进化 (ALE) 来增强西洛斯和小麦水解剂的生长.
通过过度表达大肠杆菌的跨酶基因 (pntAB) 来优化FAL生物合成.

主要成果:

工程C. glutamicum在初始瓶培养中从葡萄糖中产生了0.54g的FAL L-1.
ALE在西洛斯上提高了三倍的生长率,使小麦水解剂的高效生长成为可能.
在养批量种植中,表现最好的菌株获得了2.45g FAL L-1的产量为0.054 Cmol Cmol-1和生产率为0.109g FAL L-1h-1.
从小麦水解盐中成功地同时利用葡萄糖和西洛斯用于FAL生产.

结论:

结合代谢工程和ALE策略,使C. glutamicum从小麦水解液中有效地生产FAL.
这项研究提供了一个强大的微生物平台,用于从红细胞纤维素生物质中可持续生产FAL.
已确立的代谢工程原理可以应用于将C. glutamicum定制为第二代原料中的其他有价值产品.

关键词:

适应性实验室进化的演变.在Corynebacterium glutamicum的研究中,我们发现在 DPA 中,DPA 是 DPA 的代码.脂肪酸是一种脂肪酸.脂肪酒精是一种脂肪醇.在L-Glutamate的基础上.在L-lysine的使用中.这个名字是NMePhe.小麦的水解化合物.克西洛斯 (Xylose) 是一种酶.

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