Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Translation01:31

Translation

15.0K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
15.0K
The Nucleolus02:55

The Nucleolus

8.9K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.9K
Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

84
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
84
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

10.7K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.7K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

ChatEMG: Synthetic Data Generation to Control a Robotic Hand Orthosis for Stroke.

IEEE robotics and automation letters·2024
Same author

Therapeutic potentials of adoptive cell therapy in immune-mediated neuropathy.

Journal of autoimmunity·2024
Same author

RNA silencing of GM-CSF in CAR-T cells reduces the secretion of multiple inflammatory cytokines.

Investigational new drugs·2023
Same author

Combination of Expanded Allogeneic NK Cells and T Cell-Based Immunotherapy Exert Enhanced Antitumor Effects.

Cancers·2023
Same author

Therapeutic Potential of TNFα and IL1β Blockade for CRS/ICANS in CAR-T Therapy <i>via</i> Ameliorating Endothelial Activation.

Frontiers in immunology·2021
Same author

Cooperation of Dnmt3a R878H with Nras G12D promotes leukemogenesis in knock-in mice: a pilot study.

BMC cancer·2019

相关实验视频

Updated: Jul 23, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.4K

DNMT3A突变通过NAM-NAD代谢重编程促进白血病的发展.

Xuejiao Yang1, Xiao Wang1, Ying Yang1

  • 1Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

Journal of translational medicine
|July 18, 2023
PubMed
概括

在DNMT3A的突变驱动急性髓性白血病 (AML) 通过调节NAMPT,破坏NAD代谢和加速细胞循环. 向NAMPT为具有DNMT3A突变的AML提供了潜在的代谢疗法.

关键词:
在AML,AML就是AML.在DNMT3A突变中.代谢重编程是一种代谢重编程.NAM-NAD代谢的代谢方式这个名字叫做NAMPT.

更多相关视频

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.3K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.6K

相关实验视频

Last Updated: Jul 23, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
11:15

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

24.4K
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

8.3K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.6K

科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 代谢重编程 代谢重编程

背景情况:

  • DNA甲基转移酶3A (DNMT3A) 突变,特别是Arg882His (R882H),在急性髓性白血病 (AML) 中很常见.
  • 通过控制细胞生长和分化的基因的低甲基化,DNMT3A突变与白血病发生有关.
  • 这项研究调查了DNMT3A R882H通过代谢重编程在AML进展中的作用.

研究的目的:

  • 阐明DNMT3A R882H在AML恶性进展中的功能.
  • 调查DNMT3A突变与AML中的代谢重编程之间的联系.
  • 探索NAMPT作为一个潜在的治疗点在AML与DNMT3A突变.

主要方法:

  • 使用UHPLC-HRMS/MS进行Dnmt3a突变的小鼠血清代谢物分析.
  • 在小鼠骨髓细胞中使用MeDIP-seq和RNA-seq进行DNA甲基化和基因表达造型.
  • 分析了人类TCGA和GO数据库,进行了共免疫沉,免疫阻塞和流细胞计.
  • 在活体中使用AML小鼠模型评估NAMPT抑制功效.

主要成果:

  • DNMT3A突变导致通过DNA低甲基化增加NAMPT表达,改变NAM-NAD代谢并加速细胞周期进展.
  • 抑制NAMPT降低了环素-CDK结合,增强了CDK抑制剂相互作用,并在过度表达NAMPT的细胞中表现出更高的敏感性.
  • 向NAMPT显著改善了生存率,并在体内减少了瘤细胞透.

结论:

  • DNMT3A突变促进NAMPT的过度表达,重编程NAM-NAD代谢,并导致AML异常扩散.
  • 这种代谢重编程为DNMT3A突变的AML患者提供了潜在的治疗策略.
  • 准NAMPT为AML治疗提供了一个有前途的代谢方法.