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在体外细胞培养研究的系统审查中使用的批判性评估工具:一项方法学研究

Victor Paiva Barbosa¹, Bruna Bastos Silveira¹, Juliana Amorim Dos Santos¹

¹University of Brasília, Laboratory of Oral Histopathology, Department of Dentistry, Faculty of Health Sciences, University of Brasília, Brasília, Brazil.

Research synthesis methods

|July 18, 2023

概括

细胞培养研究的系统审查经常使用不适当的评估工具,导致方法质量差. 开发一个经过验证的工具对于改善临床前研究和基于证据的实践至关重要.

科学领域:

临床前研究方法学
在体外试验研究评估评估.
系统性质量审查质量的系统性审查.

背景情况:

临床前研究的系统审查 (SRs),特别是体外细胞培养研究,方法质量不佳.
缺乏针对细胞培养研究的验证批判性评估工具,阻碍了对临床前研究质量的评估和改进.

研究的目的:

识别和分析用于细胞培养研究SRs的现有关键评估工具.
为在体外细胞培养研究量身定制的,经过验证的批判性评估工具的开发提供信息.

主要方法:

系统地搜索和选SRs,范围审查和元分析,包括细胞培养研究和批判性评估工具.
提取使用的评估工具的数据,然后对方法质量 (MQ) 和其与研究特征的关联进行统计分析.

主要成果:

分析了82项研究,确定了32种不同的评估工具,其中约60%不是专门为细胞培养设计的.
最常使用的仪器是SYRCLE,OHAT,Cochrane的工具,GRADE,CONSORT和ToxRTool. 这些都是最常用的仪器.
在低MQ和专注于慢性退行性疾病的研究之间发现了显著的关联 (OR=5.00).

结论:

在细胞培养研究的SR中,目前的评估工具往往不足,影响研究质量.
这些发现为开发用于细胞培养研究的特定,验证的批判性评估工具提供了基础.
在临床前研究中加强批判性评估将提高基于证据的发现的可靠性和应用.

关键词:

细胞培养培养的细胞培养.批判性评价工具是一个批判性评价工具.基于证据的医学是基于证据的医学.在体外研究中的研究.方法学研究研究方法学研究.

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