在一个联盟中,植物有益的微生物之间的相互作用:Streptomyces microflavus和Trichoderma harzianum
Maria Isabella Prigigallo1, Alessia Staropoli2,3, Francesco Vinale4
1Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche, Bari, Italy.
Microbial biotechnology
|July 19, 2023
概括
构建微生物联盟是复杂的. 在体外Streptomyces microflavus (AtB-42) 和Trichoderma harzianum (M10) 之间的对抗性转向了植物中的协同作用,增强了番茄苗木的抵抗力.
科学领域:
- 微生物生态学 微生物生态学
- 植物与微生物的相互作用
- 合成生物学 合成生物学
背景情况:
- 微生物联盟提供协同效益,但构建它们是具有挑战性的.
- 菌株的相容性和相互作用需要进行彻底的研究.
- 试管中的对抗性并不总是预测植物中的行为.
研究的目的:
- 调查Streptomyces microflavus AtB-42和Trichoderma harzianum M10.0之间的相互作用. 这两种物质之间存在什么相互作用?
- 确定它们与财团开发的兼容性.
- 了解它们在体外和植物中的相互作用背后的分子机制.
主要方法:
- 在体外测定 (亚格板,液体共同培养).
- 在番茄苗木的植物研究中.
- 转录组和代谢组分析.
主要成果:
- 在AtB-42和M10.0之间观察到强烈的体外交叉对抗性.
- 在两种菌株中,共同培养诱导了显著的转录和代谢重编程.
- 在土壤中,M10的生长被AtB-42.2部分抑制.
- 与联合体接种的西红苗,与单个接种剂相比,显示出增强的防御反应.
- 在植物内协同作用被观察到,尽管在体外的对抗性.
结论:
- 在体外测试不足以预测微生物联盟的表现.
- 在植物和分子分析对于理解BCA相互作用至关重要.
- AtB-42和M10联盟证明了植物保护方面的协同作用潜力.
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