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线粒体基因组编码的长非编码RNA细胞染色体B和线粒体功能障碍在糖尿病视网膜病变

Ghulam Mohammad¹, Jay Kumar¹, Renu A Kowluru¹

¹Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University, Detroit, Michigan, USA.

Antioxidants & redox signaling

|July 19, 2023

概括

长非编码RNAB细胞染色体 (LncCYTB) 的下调会在糖尿病视网膜病变中恶化线粒体功能障碍. 恢复LncCYTB水平可以通过维持线粒体呼吸来预防糖尿病视网膜病变.

科学领域:

线粒体生物学线粒体生物学
糖尿病并发症糖尿病并发症
在RNA生物学,RNA生物学.

背景情况:

线粒体功能障碍是糖尿病并发症 (如视网膜病变) 的关键因素.
在糖尿病视网膜病变中,线粒体DNA (mtDNA) 损伤会影响电子运输链,减少细胞染色体B (CYTB) 和长非编码RNA细胞染色体B (LncCYTB) 的表达.

研究的目的:

调查LncCYTB在调节糖尿病视网膜病变中的CYTB和线粒体功能的作用.
探索LncCYTB作为糖尿病视网膜病变的潜在治疗点.

主要方法:

使用人类视网膜内皮细胞和糖尿病小鼠模型.
操纵的LncCYTB水平 (过度表达/沉默) 和暴露在高葡萄糖中的细胞.
评估了LncCYTB-CYTB相互作用,CYTB基因表达,复合III活性,线粒体活性氧物种和氧气消耗率 (OCR).

主要成果:

高葡萄糖降低了LncCYTB-CYTB相互作用和CYTB转录.
在高葡萄糖条件下,LncCYTB过度表达改善了线粒体功能并减少了氧化应激.
LncCYTB沉默加剧了葡萄糖诱导的线粒体功能障碍.
在糖尿病小鼠中观察到类似的效果,恢复线粒体平衡改善了LncCYTB-CYTB相互作用和线粒体功能.

结论:

在高血糖症中LncCYTB下调会损害CYTB转录,导致线粒体功能障碍和糖尿病视网膜病变中的OCR降低.
保持LncCYTB水平可能是通过保持线粒体呼吸来预防糖尿病视网膜病变的进展的策略.

关键词:

在LncCytBB上使用.复杂的III复杂的III复杂的细胞染色体B 细胞染色体B 糖尿病视网膜病变糖尿病视网膜病变长非编码RNA是什么意思线粒体中的线粒体.呼吸系统呼吸系统呼吸系统

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