相关实验视频

Updated: Jul 23, 2025

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities
09:10

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Published on: May 27, 2015

6.7K

查佩罗宁GroEL的水解ortho-尼特罗基β-银酸

P K Hashim1,2, Hisham M Dokainish3, Nobuyuki Tamaoki1,2

  • 1Research Institute for Electronic Science, Hokkaido University, Kita20, Nishi 10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan. hashim@es.hokudai.ac.jp.

Organic & biomolecular chemistry
|July 19, 2023
PubMed
概括

查佩罗宁GroEL在意想不到地将正-尼特罗芬β-银化物 (ONPG) 化,这是典型的 β-银化物酶基质. 分子对接表明ONPG与GroEL的ATP位点结合,揭示了一种新的酶活性.

更多相关视频

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization09:19

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Published on: March 16, 2020

7.0K
Synthesis of an Intein-mediated Artificial Protein Hydrogel15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

12.2K

相关概念视频

Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.8K
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
18.0K