野猪精液微生物组的特征和与精子质量参数的关联
Brooke E McAnally1, Molly S Smith1, Jeffrey G Wiegert1
1Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA.
Journal of animal science
|July 19, 2023
概括
猪精中的细菌群落会影响精子质量和生育能力. 这项研究将特定的细菌群体和多样性与运动性和寿命联系起来,为改善精液保存提供了洞察力.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 生殖生物学 生殖生物学
背景情况:
- 猪精中的细菌污染会降低精子的寿命和生育能力.
- 了解野猪精液微生物组对于优化人工授精 (AI) 实践至关重要.
研究的目的:
- 用16S rRNA测序来描述野猪精液中的细菌群体.
- 为了将微生物种群的流行率和多样性与精子质量参数的时间和研究之间的相关性.
主要方法:
- 在精液样本上进行了V4高变区的16S rRNA基因测序.
- 在5天的时间内评估了精子的运动性和凝结性.
- 分析了α和β多样性指标,并与精子参数相关联.
主要成果:
- 猪 (A和B) 之间的细菌群体有显著差异,并在5天的收集期间发生了变化.
- 与B种子相比,A种子的精子凝结率更高,细菌的丰度不同 (蛋白质细菌,菌细菌,细菌细菌,细菌细菌,行动细菌).
- 随着时间的推移,精子运动性下降,与细菌多样性指数 (Chao1,Shannon) 和特定属 (Prevotella,Ruminococcus,Bacteroides) 有负相关.
结论:
- 猪精液中的细菌群体组成和多样性与精子质量有关.
- 种子之间的微生物组的变化和随着时间的推移,可以影响精子的寿命和生育能力.
- 这些发现强调了管理精液微生物群对于改善AI结果的重要性.
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