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男性化基因控制了Hyphantria cunea中的性分化
Xiaowei Li1,2, Huihui Liu3, Honglun Bi2
1Laboratory of Evolutionary and Functional Genomics, School of Life Sciences, Chongqing University, Chongqing, China.
Insect science
|July 19, 2023
概括
这种Hyphantria cunea Masculinizer (HcMasc) 基因对男性和女性的性发育和剂量补偿至关重要. 破坏HcMasc会影响性别比率和生殖器官,为侵入性害虫控制提供潜力.
科学领域:
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 雄性化基因 (Masc) 控制了Bombyx mori.的性别决定.
- 虫 (Hyphantria cunea) 是一个重要的入侵性森林害虫.
- 了解H. cunea中的Masc功能对于害虫控制策略至关重要.
研究的目的:
- 为了识别和功能性地描述Hyphantria cunea.中的Masc基因 (HcMasc).
- 研究HcMasc在该物种的性别确定和剂量补偿中的作用.
主要方法:
- 对HcMasc基因进行序列分析.
- 跨发育阶段和组织的表达特征.
- 在CRISPR/Cas9中介的HcMasc外形1和3中发生基因破坏.
主要成果:
- HcMasc与B. mori Masc.共享保留的域 (CCCH型指,核定位信号,男性确定域)
- 与B. mori不同,HcMasc在两性和不同发育阶段都表现出无处不在的表达.
- HcMasc的破坏导致了男性和女性H. cunea. 的性别比率偏差和异常的生殖器官.
结论:
- 在H. cunea. 中,HcMasc基因对于男性和女性的性差异化和剂量补偿至关重要.
- 这些发现为开发针对H. cunea. 的新型害虫控制方法提供了基础.
- 这项研究突出了类鸟物种之间Masc基因的功能分歧.
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