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Updated: Jul 23, 2025

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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用目标生物分子修改细菌微分区通过后翻译SpyTagging通过

David M Beal1, Mingzhi Liang1, Ian Brown1

  • 1School of Biosciences, Division of Natural Sciences, University of Kent Canterbury UK d.m.beal@kent.ac.uk c.m.smales@kent.ac.uk.

Materials advances
|July 19, 2023
PubMed
概括

研究人员开发了一种新的方法,使用后翻译修改来修改细菌微分区 (BMC). 这种技术使得BMC上的蛋白质表面显示成为潜在的疫苗开发和药物输送应用.

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相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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