GLP-1激动剂,exendin-4,刺激雌羊的LH分泌
Elizabeth M Simpson1,2, Iain J Clarke3, Christopher J Scott4
1School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, NSW, Australia.
The Journal of endocrinology
|July 19, 2023
概括
作为一种类似于葡萄糖-1 (GLP-1) 受体激动剂的exendin-4的全身治疗,可以刺激雌羊的黄素化激素 (LH) 分泌. 这表明从后肠内源性GLP-1可能系统调节LH释放.
科学领域:
- 生殖内分泌学 生殖内分泌学
- 胃肠道生理学 胃肠道生理学
背景情况:
- 之前的研究表明,直接微注射葡萄糖类-1 (GLP-1) 或exendin-4到绵羊中部突出部刺激黄素激素 (LH) 分泌.
- 系统性给予GLP-1受体激动剂对绵羊LH分泌的作用仍未得到研究.
研究的目的:
- 为了确定系统静脉注射 (IV) 输注exendin-4能否刺激雌羊的LH分泌.
- 调查雌激素周期阶段对LH对埃克森丁-4反应的影响.
- 检查exendin-4对血胰岛素和葡萄糖水平的影响,并确认羊胃肠道中葡萄糖基因 (GCG) 的表达.
主要方法:
- 绵羊在卵泡阶段被静脉注射0.5毫克或2毫克的exendin-4或载体,持续1小时.
- 在输液前,输液期间和输液后,每隔10分钟测量血LH度.
- 使用定量PCR分析胃肠道样本中的GCG基因表达.
主要成果:
- 两个剂量的exendin-4显著增加了平均血LH度和LH外围脉冲幅度.
- 施用Exendin-4并没有影响LH脉冲间隔或排卵前LH激增的时间.
- 血胰岛素和葡萄糖水平保持不变,但GCG基因表达在后肠中得到证实,向直肠增加.
结论:
- 系统性给予exendin-4刺激雌羊的LH分泌,这表明系统性作用GLP-1的潜在作用.
- 内生GLP-1,可能源于后肠,可能在刺激LH分泌中发挥生理作用.
- 激发LH的有效剂量似乎低于因因克雷丁效应所需的有效剂量.
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