相关实验视频

Updated: Jul 23, 2025

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Published on: April 23, 2019

使用表达嗅觉受体的细胞阵列,暂时改变气相气味混合物的积极追踪

Hongchao Deng¹, Yuji Sukekawa², Hidefumi Mitsuno²

¹Laboratory for Future Interdisciplinary Research of Science and Technology, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori, Yokohama, Kanagawa 226-8503, Japan.

Analytical chemistry

|July 19, 2023

概括

这项研究引入了一种新的气相气味生物传感器,使用嗅觉受体 (ORs) 来实时跟踪不断变化的气味混合物. 生物传感器有效地区分和量化复杂的气味,克服了以前液相传感器的局限性.

科学领域:

生物技术是生物技术.
化学传感器化学传感器
嗅觉受体技术嗅觉受体技术

背景情况:

嗅觉受体 (ORs) 对于嗅觉检测至关重要,并在生物传感器中广泛使用.
现有的生物传感器仅限于检测静态,单元,液相气味剂.
需要能够实时分析动态气相气味混合物的生物传感器.

研究的目的:

开发一种先进的气味生物传感器,使用一组OR表达细胞来积极跟踪暂时变化的气相气味混合物.
为了实现复杂的气味环境的实时监测和量化.
为了克服当前气味检测技术的局限性.

主要方法:

利用表达嗅觉受体 (ORs) 的细胞阵列,包裹在薄而稳定的液体膜中,用于气相气味的透.
采用在线图像处理来获得生物传感器响应计算的单个细胞亮度数据.
开发了调整已知气味成分以匹配未知气味的方法,并通过可变单元和二元气味混合物验证了性能.

主要成果:

生物传感器在气相中成功跟踪了可变单元气味和二元气味混合物.
建立了一种方法,通过比较相邻的未知和已知的气味反应来克服传感器漂移.
标签前方法和无标签方法都被证明对气味混合物定量有效,无标签方法尽管具有OR气味交叉敏感性,但仍显示出能力.

结论:

开发的基于OR的生物传感器可以积极跟踪动态气相气味混合物.
该系统有效量化气味成分,并减轻传感器漂移.
这项技术为复杂环境中的实时气味分析提供了重大进步.

更多相关视频

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Published on: October 2, 2017

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Published on: July 13, 2015

相关概念视频

Olfaction

Olfaction

The sense of smell is achieved through the activities of the olfactory system. It starts when an airborne odorant enters the nasal cavity and reaches olfactory epithelium (OE). The OE is protected by a thin layer of mucus, which also serves the purpose of dissolving more complex compounds into simpler chemical odorants. The size of the OE and the density of sensory neurons varies among species; in humans, the OE is only about 9-10 cm2.
The olfactory receptors are embedded in the cilia of the...

Physiology of Smell and Olfactory Pathway

Physiology of Smell and Olfactory Pathway

Humans detect odors with the help of specialized cells located in the upper part of the nasal cavity, called olfactory receptor neurons (ORNs). ORNs possess hair-like structures called cilia, which are receptive to sensations from the inhaled air. When an odorant molecule binds to a specific receptor on the cell of the cilia, it leads to a series of events that ultimately cause the ORN to send electrical signals to the olfactory bulb in the brain through the olfactory nerves.
The olfactory...

Olfactory Receptors: Location and Structure

Olfactory Receptors: Location and Structure

The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...