同时生产红醇和β-胡卜素的工程
Shuo Xu1, Xinyi Zhang1, Yue Zhang1
1State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Journal of agricultural and food chemistry
|July 19, 2023
概括
这项研究设计了酵母共同产生红醇和β-胡卜素,提高了酵母细胞的价值. 开发的菌株实现了这两种化合物的高产量,为生产有价值的烯提供了一种具有成本效益的方法.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 红醇生产产生了丰富的酵母生物质,利用率有限.
- β-胡卜素是一种高价值化合物,可以由工程微生物合成.
- 开发联合生产平台可以提高生物工艺的经济可行性.
研究的目的:
- 设计一种能够共同生产红醇和β-胡卜素的酵母菌株.
- 为了提高酵母生物质的价值从红醇生产.
- 建立一种成本效益高的方法来生产疏水性烯酸.
主要方法:
- 评估了具有不同形态的生产红醇的酵母菌株.
- 通过删除Mhy1和Cla4基因,构建了一个类似酵母的形态底盘.
- 引入了β-胡卜素合成基因 (CarRA,CarB) 和过度表达的速度限制基因 (GGS1,tHMG1).
- 通过过度表达YTH1和YTH3基因进行传送工程,以减轻发酵延迟.
主要成果:
- 成功构建了一个β-胡卜素和红醇联合生产的酵母菌株 (ylmcc).
- 通过基因过度表达,实现了45.32倍的β-胡卜素产量增加.
- 通过工程传送器优化发酵,减轻由β-胡卜素积累引起的延迟.
- 在批量发酵中产生了171 g/L的红醇和47.36 ± 0.06 mg/g的DCWβ-胡卜素.
结论:
- 工程酵母菌株有效地共同生产红醇和β-胡卜素.
- 这种方法提高了生产红醇的酵母生物质的价值.
- 提供一种低成本的生物技术途径,用于生产有价值的疏水性类.
关键词:
雅罗维亚 (Yarrowia lipolytica) 是一个高脂的植物.这是一部联合制作的作品.两形态主义二形态主义红醇 (Erythritol) 是一种红醇.乙-胡卜素 β-胡卜素 的含量.更多相关视频
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