在体外成熟介质中补充普特瑞辛对牛胚胎发育和质量的影响
Esra Bicici1, Fatma Satilmis1, Mustafa Bodu2
1Department of Obstetrics and Gynecology, Selcuk University, Konya, Turkey.
Animal biotechnology
|July 19, 2023
概括
在牛体外胚胎生产期间补充普特瑞辛,显著改善了卵细胞成熟,积分扩张和胚胎发育. 这种方法增强了化恢复,并增加了胚胎细胞的细胞数量,以提高胚胎质量.
科学领域:
- 生殖生物学 生殖生物学
- 胚胎学 胚胎学
- 动物科学动物科学
背景情况:
- 试管胚胎生产 (IVEP) 对畜牧业和研究至关重要.
- 卵细胞成熟是影响后续胚胎发育的关键阶段.
- 聚氨酸,如素,在细胞过程中发挥作用,但它们对IVEP的影响尚未完全理解.
研究的目的:
- 研究在成熟介质中补充普特瑞辛对牛卵细胞成熟的影响.
- 在IVEP期间评估普特雷辛对胚胎发育和质量的影响.
- 为了评估putrescine对累积膨胀和半变异恢复率的影响.
主要方法:
- 荷尔斯坦牛卵细胞 (I类) 被分为两组:补充普特雷辛 (0.5毫米) 和对照组.
- 卵子经历了体外成熟,随后是受精和体外培养.
- 评估了累积体扩张,半分裂恢复,裂变率,胚胎细胞发育和细胞计数 (内部细胞质量,体,总数).
主要成果:
- 普特瑞辛组显示显著更高的III级累积扩张率 (74.21%vs. 60.4%) 和较低的I级扩张率 (11.95%vs. 26.17%).
- 随着累积体的扩张,中症复发率有所增加,而雷素补充剂增强了这种相关性.
- 布特雷辛组的裂变率和芽细胞形成显著高于布特雷辛组 (p < .05),内细胞质量,体和总细胞数量增加.
结论:
- 在成熟介质中补充普特瑞辛可增强牛IVEP期间的累积膨胀和半变异恢复.
- 添加普特雷辛对卵细胞发育能力产生了积极的影响,从而改善了胚胎细胞的数量和质量.
- 普特雷辛是优化牛体内胚胎生产的有希望的补充剂.
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