几种阿尔法疹病毒与NF-κB通路相似地相互作用,并抑制NF-κB依赖基因表达
Nicolás Romero1,2, Alexander Tishchenko1, Ruth Verhamme1
1Department of Translational Physiology, Infectiology and Public Health-Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium.
Microbiology spectrum
|July 19, 2023
概括
阿尔法疹病毒抑制宿主病毒.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 阿尔法疹病毒感染减弱了宿主在粘膜表皮上的天生的免疫力.
- 核因子kappa B (NF-κB) 途径对于先天免疫反应至关重要.
- NF-κB驱动炎症反应,这对于控制病毒感染至关重要.
研究的目的:
- 为了研究不同的alphaherpesvirus如何与表皮细胞中的NF-κB通路相互作用.
- 为了比较各种alphaherpesvirus用来调节NF-κB驱动的免疫反应的策略.
- 为了确定NF-κB抑制是否是alphaherpesvirus中保存的机制.
主要方法:
- 以多个alphaherpesvirus (HSV-1,HSV-2,FHV-1,BoHV-1) 感染上皮细胞系的感染.
- 对NF-κB通路激活和转录活动的分析.
- 对NF-κB依赖基因表达的评估,包括IκB.
主要成果:
- 几种阿尔法疹病毒,包括HSV-1,HSV-2和FHV-1,通过抑制转录,使NF-κB驱动的反应失活,尽管NF-κB持续激活.
- 牛牛疹病毒1 (BoHV-1) 不同,在感染细胞中没有持续的NF-κB激活.
- 所有测试的病毒都阻止了IκB的表达,这是一个关键的NF-κB依赖基因.
结论:
- 抑制NF-κB转录活性是多种alphaherpesvirus用来逃避宿主天生的免疫力的常见策略.
- 在感染的上皮细胞中抑制NF-κB依赖基因表达是alphaherpesvirus病变发生的一个重要方面.
- 病毒基因组复制似乎是必要的,以抑制大多数研究的alphaherpesvirus的NF-κB依赖转录.
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