来自虫相关的真菌Penicillium chrysogenum的抗菌代谢物
Tian-Xiao Li1, Hai-Yang Su1, Jian-Chun Yu2
1College of Food and Biological Engineering, Zhengzhou University of Light Industry, 136 Kexue Road, Zhengzhou 450001, China.
在真菌Penicillium chrysogenum中发现了抗菌化合物,这是甲虫Aspongopus chinensis的共生物. 这些代谢物对阳性细菌表现出强烈的活性,为新的抗菌疗法提供了潜力.
科学领域:
- 微生物学 微生物学
- 自然产品 化学 化学
- 化学生态化学生态学
背景情况:
- (Penicillium chrysogenum) 的真菌与甲 (Aspongopus chinensis) 的甲虫有关.
- 菌是众所周知的具有生物活动的各种二次代谢物的生产者.
- 研究共生关系可以发现新的生物活性化合物.
研究的目的:
- 为了隔离和识别与阿斯庞戈普斯chinensis相关的Penicillium chrysogenum的抗菌二次代谢物.
- 评估分离化合物的抗菌活性.
- 为了建立真菌和甲虫之间的共生关系.
主要方法:
- 菌种的乙酸乙烯提取物经过了染色分离.
- 使用质谱学和核磁共振 (NMR) 谱学阐明了化合物结构.
- 进行最小抑制度 (MIC) 测试以确定抗菌活性.
主要成果:
- 他们分离了五种化合物:埃莫丁,4-甲甲基) 酸,异甲酸,甲酸D和迪塞兰德罗尔A.
- 埃莫丁对黄金葡萄球菌 (MIC = 6.3μg/mL) 显示出强烈的活性.
- 模态四二桑 (二酸D,二兰德A) 显示出强烈的活性 (MICs=9.528.5μg/mL).
结论:
- Penicillium chrysogenum首次被确定为甲虫Aspongopus chinensis的一种共生真菌.
- 这种真菌菌株产生强大的抗菌二次代谢产物.
- 隔离的化合物显示出显著的潜力,以抑制在体外Gram阳性细菌的生长.
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