使用Reactome-IDG网页门户揭示暗蛋白的功能
Deidre Beavers1, Timothy Brunson1, Nasim Sanati1
1Oregon Health & Science University, Portland, Oregon.
Current protocols
|July 19, 2023
概括
反体-IDG项目通过将它们整合到策划的途径中,提高对研究不足的蛋白质的理解. 这有助于发现新的治疗点和疾病机制.
科学领域:
- * 生物信息学是一门学科.
- * 系统生物学 系统生物学
- * 药物发现 药物发现
背景情况:
- * 缺乏研究或"暗"蛋白质为发现新的分子机制和疾病治疗点提供了潜力.
- *Reactome-IDG项目利用Reactome,一个全面的通路知识库,来对这些暗蛋白进行上下文化.
- * 了解暗蛋白的功能对于推进精准医学和识别新药点至关重要.
研究的目的:
- * 介绍Reactome-IDG网络门户,用于探索暗蛋白在生物通路中的功能性作用.
- * 提供使用门户预测蛋白质功能和治疗潜力的协议.
- * 促进暗色蛋白质融入现有的生物通路知识中.
主要方法:
- * 开发和部署Reactome-IDG网络门户网站 (https://idg.reactome.org).
- * 黑色蛋白质集成到Reactome手动策划的路径中.
- *利用增强的可视化工具,包括系统生物学图表符号 (SBGN) 和功能交互 (FI) 网络.
- * 覆盖组织特异性表达数据,药物向相互作用和蛋白质/基因关系.
主要成果:
- *Reactome-IDG门户网站可以交互搜索与暗蛋白相关的通路.
- * 用户可以通过检查它们在Reactome路径中的相互作用来预测蛋白质功能.
- * 增强的可视化允许对功能上下文进行详细的研究,包括组织特异性和分子相互作用.
结论:
- *Reactome-IDG项目为研究研究不足的蛋白质的研究人员提供了宝贵的资源.
- * 网络门户网站有助于预测暗蛋白的功能和潜在的治疗应用.
- *将暗色蛋白质整合到通路知识中,加速了新生物机制和药物点的发现.
相关概念视频
Protein Networks
4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K
Protein-protein Interfaces
12.6K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.6K
Proteomics
7.4K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.4K
Covalently Linked Protein Regulators
1.7K
1.7K
Protein Families
15.4K
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism. Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members. If these new proteins contain similar amino acids in key...
15.4K
Tagging and Fusion Proteins
6.7K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.7K


