相关实验视频

Updated: Jun 17, 2026

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate [18F]SFB
08:33

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate [18F]SFB

Published on: June 28, 2011

13.0K

在有氧条件下使用重组SUF系统合成 [4Fe-4S] 蛋白质

Po-Hsiang Wang1,2, Shota Nishikawa3,4, Shawn Erin McGlynn3,5

  • 1Department of Chemical Engineering and Materials Engineering, National Central University, Taoyuan 32001, Taiwan.

ACS synthetic biology
|July 19, 2023
PubMed
概括

研究人员开发了一种新方法,在无细胞系统中生产活性铁硫 (Fe-S) 蛋白质. 这一突破使必要的 [4Fe-4S] 蛋白质的有氧合成成为可能,进步了合成生物学和生物催化.

关键词:
在Fe−S集群中,在 SUF 中,辅助蛋白质是 SUF 的辅助蛋白质.亚可尼特酶是一种辅助因子再生的复原复制的无细胞蛋白质合成.氧化还原酶 氧化还原酶

更多相关视频

OnePot PURE Cell-Free System08:25

OnePot PURE Cell-Free System

Published on: June 23, 2021

8.8K
Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus06:45

Author Spotlight: Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Published on: June 2, 2023

2.2K

相关概念视频

Formation of Complex Ions03:45

Formation of Complex Ions

A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
Sulfur Assimilation01:20

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...