理论和实验研究植物依赖光的原烯酸氧化还原酶作为寻找潜在除草剂的新目标
Ruiyuan Liu1, Leng Wang1, Yue Meng1
1College of Science, China Agricultural University, Beijing 100193, China.
Journal of agricultural and food chemistry
|July 19, 2023
概括
这项研究引入了一种新的除草剂作用模式,向依赖光的酶NADPH:原基氧降解酶 (LPOR). 研究人员发现了新的LPOR抑制剂,有可能在农业中进行选择性和安全的杂草控制.
科学领域:
- 农业科学 农业科学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 抗除草剂耐药性是影响作物生产的重大全球挑战.
- 这种依赖光的酶NADPH:protochlorophyllide oxidoreductase (LPOR) 是新型除草剂的潜在代谢标.
研究的目的:
- 提出和验证一种针对LPOR的新型除草剂作用模式.
- 识别和描述具有选择性和安全杂草控制潜力的新型LPOR抑制剂.
主要方法:
- 基于结构的虚拟选,针对多个LPOR绑定站点.
- 化合物的生物活性测定,包括IC50测量.
- 在Arabidopsis thaliana中进行转录组分析和分子动力学模拟.
主要成果:
- 对LPOR进行微分子抑制 (IC50 = 4.74 μM) 的化合物2的鉴定.
- 组合了2个参与叶绿素合成的上调基因,特别是LPOR基因.
- 分子动力学模拟表明,在抑制剂结合后,稳定性和刚性得到了增强.
结论:
- 这项研究为开发针对LPOR的除草剂提供了一个新的策略.
- 这些发现为设计针对抗除草剂耐药杂草的有效抑制剂提供了指导方针.
- LPOR 抑制剂在农业中对选择性和安全的杂草管理有希望.
相关概念视频
Photoreceptors and Plant Responses to Light
20.5K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
20.5K
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
The Z-Scheme of Electron Transport in Photosynthesis
10.3K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
10.3K
The Calvin Benson Cycle
4.6K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.6K
The Photochemical Reaction Center
4.2K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.2K
Channel Rhodopsins
2.6K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.6K


