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通过功能性元基因组学发现了两个双功能/多功能细胞体

Lingzhi Hu¹, Shengxia Zhang¹, Shumao Chai¹

¹College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.

Enzyme and microbial technology

|July 19, 2023

概括

来自土壤转基因组学的两种新型细胞质对生物燃料应用有希望. ZF994-1和ZF994-2表现出明显的酶活性和良好的稳定性,表明了工业潜力.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
微生物生态学微生物生态学

背景情况:

细胞酶是重要的工业酶,特别是在生物燃料生产中.
超基因组方法可以从无法培养的微生物中发现新型酶.
识别具有理想性质的新细胞质对于推动生物技术发展至关重要.

研究的目的:

从土壤元基因组图书馆中识别和描述新型细胞质.
评估已识别的细胞酶的酶活性和工业适用性.
研究新型酶的稳定性和基质特异性.

主要方法:

对土壤元基因组图书馆进行选,以寻找细胞酶基因.
重组细胞酶 (ZF994-1和ZF994-2) 的基因克隆,表达和净化.
遗传学分析以确定酶家族 (GH12和GH3).
酶活性测定基质特异性和稳定性.

主要成果:

确定了编码细胞质ZF994-1 (GH12) 和ZF994-2 (GH3) 的两个双联基因.
ZF994-1显示了内葡萄糖酶,β-1,3-葡萄糖酶,β-1,4-曼酶和强大的β-葡萄糖酶活性.
ZF994-2表现出中度的内葡萄糖酶和强大的β-葡萄糖酶活性.
ZF994-1表现出优异的葡萄糖耐受性 (>45%的活性保留在3M葡萄糖中).
在金属离子和有机试剂的存在下,ZF994-2表现出高活性.

结论:

鉴定到的细胞质ZF994-1和ZF994-2具有有价值的酶特性.
ZF994-1的葡萄糖耐受性和ZF994-2的稳定性表明了工业应用的巨大潜力,包括生物燃料的生物转化.
这些发现有助于发现用于生物技术进步的新型酶.

关键词:

双功能/多功能细胞质.输内葡萄糖酶是一种内葡萄糖酶.功能性元基因组学是什么不同类的表达式不同类的表达式这就是β-glucosidase.

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