氧化胆固醇暴露对结肠炎的不良影响是由肠道微生物群的调节介导的
Chi Yan1, Shou-He Huang1, Hua-Fang Ding1
1Food and Nutritional Sciences Program, School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
Journal of hazardous materials
|July 19, 2023
概括
饮食中氧化胆固醇 (OXC) 通过破坏肠道细菌,使炎症性肠病 (IBD) 恶化. 在小鼠模型中,OXC会引起肠道失调,增加大肠炎的易感性.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 在全球范围内,炎症性肠病 (IBD) 的发病率正在上升.
- 饮食中的胆固醇及其氧化形式 (OXC) 是常见的.
- 在IBD病原体中OXC的作用需要阐明.
研究的目的:
- 研究饮食中氧化胆固醇 (OXC) 促进结肠炎的机制.
- 为了利用C57BL/6小鼠模型研究OXC诱导的大肠炎.
主要方法:
- 治疗小鼠时使用硫酸 (DSS) 和/或OXC.
- 用16S rRNA测序分析了肠道微生物组成.
- 用抗生素治疗和便微生物群移植来评估微生物的作用.
主要成果:
- 在小鼠中,OXC加剧了DSS诱导的大肠炎的严重程度.
- 在健康小鼠中,短期的OXC暴露并没有直接损害肠道屏障或引起炎症.
- OXC诱导肠道失调,减少有益的短链脂肪酸 (SCFA) 生产细菌,增加潜在的有害细菌.
- 抗生素治疗取消了OXC诱导的大肠炎恶化,涉及肠道微生物群.
- 从暴露于OXC的小鼠中与异生菌微生物群的殖民化诱导了无菌接受者的严重结肠炎.
结论:
- 饮食中氧化胆固醇 (OXC) 增加了对结肠炎的易感性.
- 通过诱导肠道微生物群失调,OXC会加剧结肠炎.
- 由OXC介导的肠道失调是促进炎症性肠病的关键因素.
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