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鼠标Pxt1的表达受Mir6996小RNA的调节

Igor Tomczyk¹, Mikołaj Rokicki¹, Wioleta Sieńko¹

¹Department of Genetics and Evolution, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland.

|July 19, 2023

概括

鼠标Pxt1蛋白诱导细胞死亡,但由男性生殖细胞中的微RNA (miRNA) 调节. 这项研究确定了Mir6996-3p作为一个关键的调节剂,防止Pxt1介导的细胞死亡.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
基因规则基因规则

背景情况:

小鼠的Pxt1基因仅在雄性生殖细胞中表达,编码一种可以诱导细胞死亡的蛋白质.
与BAG6蛋白的相互作用通常可以防止PXT1介导的细胞死亡.
假设是Pxt1调节,可能由miRNA,防止细胞死亡在发展的男性生殖细胞.

研究的目的:

研究小鼠雄性生殖细胞中Pxt1基因的调节机制.
为了确定miRNA是否调节Pxt1的表达和功能.
为了确定参与Pxt1调节的特定miRNAs.

主要方法:

使用Pxt1淘汰和转基因小鼠过度表达Pxt1.
雇佣的ELISA测试检测成年老鼠丸中的PXT1蛋白丰富度.
在记者等离子体中使用Pxt1 3'UTR应用双Glo luciferase测定,以评估miRNA调节.
使用mirDB和局部定向突变发生来识别和确认miRNA目标.

主要成果:

在成年老鼠丸中,证明了PXT1蛋白的低丰度表达.
通过光酶记者测定证实Pxt1是由miRNA调节的.
确定了Mir6996-3p作为通过mirDB和突变发生的Pxt1翻译的抑制剂.

结论:

男性生殖细胞中的Pxt1表达是由miRNA调节的.
Mir6996-3p在抑制Pxt1翻译方面发挥着至关重要的作用.
在涉及miRNA的小鼠雄性生殖细胞中提出了Pxt1调节的模型.

关键词:

细胞死亡细胞死亡基因沉默是一种基因沉默.精子发生是精子生成.这是一个小RNARNA.

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