在三个分析平台上对薄荷薄荷基准测试实验进行交叉验证
Y Lan Pham1,2, Ruyi Yu1, Jonathan Beauchamp1
1Fraunhofer Institute for Process Engineering and Packaging IVV, Freising, Germany.
Journal of breath research
|July 19, 2023
概括
薄荷薄荷实验对挥发性烯的呼吸分析方法进行了基准比较. 高度的生物变异性,而不是仪器差异,解释了呼吸生物标志物研究中的各种研究结果.
科学领域:
- 分析化学 分析化学
- 生物标志物发现发现
- 药理动力学 药理动力学
背景情况:
- 呼吸分析缺乏生物标志物研究的相互可比性.
- 挥发性烯冲洗概况是关键指标.
- 标准化的基准测试对于可靠的结果至关重要.
研究的目的:
- 为了对呼吸分析的分析平台进行基准测试.
- 从薄荷油中特征特洗配置文件.
- 确定呼吸生物标志物研究中的变异性来源.
主要方法:
- 薄荷薄荷实验涉及11名志愿者提供三倍的呼吸样本.
- 分析使用了质子转移反应-飞行时间-质谱法 (PTR-TOFMS),气色谱法-离子移动谱法 (GC-IMS) 和热溶解综合二维气色谱法-飞行时间-质谱法 (TD-GC×GC-TOFMS).
- 呼吸样本采集使用缓冲终潮 (BET) 样本,手持直接呼吸样本和呼吸采集用于体外分析 (ReCIVA) 系统.
主要成果:
- 平均洗时间为448分钟 (PTR-TOFMS,GC-IMS) 和372分钟 (TD-GC×GC-TOFMS),与基准保持一致.
- 观察到基洗配置文件的显著个体间和个体内变异性.
- 低的仪器间变异性表明生物因素驱动结果差异.
结论:
- 生物变异性,而不是分析平台的性能,是独立呼吸生物标志物研究结果差异的主要驱动因素.
- 标准化呼吸分析协议对于一致和可比的结果至关重要.
- 薄荷薄荷实验为未来的呼吸分析研究提供了一个基准.
关键词:
GC × GC-MS 的时间在GC-IMSS中.这是一个PTR-MS.基准测试 (benchmarking) 是一种比较的方法.胡薄荷:胡薄荷是一种薄荷.药物动力学 药物动力学标准化 标准化 标准化更多相关视频
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