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Lacticaseibacillus paracasei发酵鉴定了Y2Fr及其对Vibrio parahaemolyticus的抗菌活性

Shen Yang¹, Yufan Xing¹, Jialong Gao²

¹College of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, China.

Microbial pathogenesis

|July 19, 2023

概括

一种新型抗微生物,Y2Fr,来自Lacticaseibacillus paracasei,有效地对抗大黄的Vibrio parahaemolyticus感染. 这种具有作为水产养殖中传统抗生素的安全和稳定的替代品的潜力.

科学领域:

水生动物的健康状况.
微生物学微生物学
生物技术是生物技术.

背景情况:

维亚血溶菌感染对大黄鱼 (Larimichthys crocea) 构成重大威胁.
像抗生素这样的传统治疗方法引起了人们对抗菌素耐药性和环境污染的担忧.

研究的目的:

确定和描述用于治疗V. parahaemolyticus感染的新型抗菌.
评估Y2Fr作为潜在治疗剂的疗效和安全性.

主要方法:

从Lacticaseibacillus paracasei发酵中分离,使用超高性能液体染色体质谱法.
基于电荷,疏水性和预测结构的抗微生物Y2Fr的查.
评估抗菌活性 (MIC,MBC,时间杀戮测试),作用机制 (膜相互作用,DNA结合) 和安全性 (细胞毒性,血液溶解活性,血稳定性).

主要成果:

酸Y2Fr对V. parahaemolyticus表现出强大的抗菌活性,MIC为125μg/mL,MBC为250μg/mL.
Y2Fr经历结构变化以提高膜透性,并与细菌DNA和特定蛋白质 (KKI11460.1,A0A0L8TVA4) 结合.
在生理条件下,Y2Fr表现出非细胞毒性,非溶血性质和稳定性.

结论:

酸Y2Fr是水生动物治疗和预防V. parahaemolyticus感染的有希望的候选物.
它的机制涉及膜破坏和DNA相互作用,具有有利的安全性.

关键词:

具有抗菌活性,具有抗菌活性.抗菌机制是一种抗菌机制.抗微生物是一种抗微生物.乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶乳糖酶这种病毒是Vibrio parahaemolyticus.

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