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Miryam Pando¹, Ruoting Yang², George Dimitrov³

¹US Army Institute of Surgical Research (USAISR), JBSA Ft Sam Houston, San Antonio, Texas.

The journal of pain

|July 19, 2023

概括

无法预测的战斗压力和热伤害会改变大鼠细胞外囊泡微RNA (EV-miRNAs),影响疼痛敏感性. 这些EV-miRNA可以作为战斗和操作应激反应 (COSR) 和受伤相关疼痛的生物标志物.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
压力生理学压力生理学

背景情况:

战斗和作战应激反应 (COSR) 和热损伤可以增加疼痛敏感性.
细胞外囊泡微RNAs (EV-miRNAs) 通常会抑制炎症,但在神经病痛模型中是下调的.
之前的研究表明,热损伤前不可预测的战斗压力 (UPCS) 会增加雄性大鼠的疼痛.

研究的目的:

研究UPCS和热伤害对流通的EV-miRNAs在雄性和雌性大鼠中的影响.
确定EV-miRNA是否可以作为COSR和相关机械敏感性的生物标志物.
探索COSR和热损伤引起的疼痛的潜在治疗点.

主要方法:

成年雄性和雌性Sprague Dawley大鼠被暴露在UPCS中2周或4周.
实验组包括非压力 (NS),压力 (S),NS +热损伤 (TI) 和S + TI.
测量了机械敏感性,收集了血,并将EV-miRNAs分离出来用于小RNA测序和分析.

主要成果:

仅仅暴露于UPCS就会在两种性别中诱导机械体.
在高峰UPCS期间的热损伤与单独的热损伤相比,加剧了机械体.
在压力和非压力组之间观察到EV-miRNAs的差异表达,有和没有热损伤.

结论:

在COSR和机械灵敏度的发展过程中,可以检测到EV-miRNAs的一致差异.
EV-miRNAs显示出作为COSR和热损伤引起的疼痛的关键调节器,生物标志物和治疗点的潜力.
这些在动物模型中的发现表明预后和诊断生物标志物的潜在临床相关性.

关键词:

没有感觉,没有感觉.生物标志物生物标志物细胞外囊泡中的细胞外囊泡.这是一个微型RNA.热伤害的伤害是由于热伤害造成的.

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