在Rhipicephalus microplus中抗酸盐的耐药性和采样群体中ABC载体基因的表达特征
Mukesh Shakya1, Anil Kumar Sharma2, Sachin Kumar2
1Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly- 243122, Uttar Pradesh, India; IVRI-Eastern Regional Station, 37 Belgachia Road, Kolkata 700037, West Bengal, India.
Experimental parasitology
|July 19, 2023
概括
牲畜对德尔塔米特林等化学物质的耐药性很高,对伊弗梅克丁和菲普罗尼尔的耐药性正在出现. 雌激酶的产生和一个特定的基因突变与Rhipicephalus microplus的德尔塔米特林耐药性相关.
科学领域:
- 兽医昆虫学 兽医昆虫学
- 分子昆虫学分子昆虫学
- 农药耐药性 农药耐药性是一种
背景情况:
- 化学杀剂是家畜控制的主要工具.
- 增加抗常用化学物质的耐药性,如deltamethrin需要耐药性监测.
- (Rhipicephalus microplus) 是一种占主导地位的物种,在全球范围内影响牲畜.
研究的目的:
- 为了监测Rhipicephalus microplus野外种群中的抗素耐药性.
- 研究耐药性的生物化学和分子基础.
- 为有效的管理策略提供信息.
主要方法:
- 从中邦的11个地区和旁遮普的一个地区收集Rhipicephalus microplus.
- 使用成人浸泡和幼虫包装测试对deltamethrin,cypermethrin,coumaphos,ivermectin和fipronil进行酸盐敏感性测试.
- 对雌激酶,单氧激酶和谷氨酸S转移酶活性进行生物化学测试.
- 分子分析包括通道基因测序和基因表达研究 (RmABCC2,RmABCC1,RmABCB10).
主要成果:
- 在特定隔离物中对德尔塔米特林 (RF 3.98-38.84) 的高耐药性和对赛珀米特林的中等耐药性.
- 在所有隔离物体中观察到对伊弗梅克丁 (RF 1.05-4.98) 和菲普罗尼尔 (RF 0.40-2.18) 的新兴耐药性.
- 升高的雌激酶产生与德尔塔米特林耐药性 (p < 0.01) 有显著的相关性 (90%).
- 通道基因中的C190A点突变与德尔塔美林耐药性有关.
- 甲治疗导致RmABCC2基因的上调,这表明它在甲耐药性中起着作用.
结论:
- 瑞皮塞法卢斯微普拉斯种群对德尔塔米特林表现出显著的耐药性,对其他虫杀虫剂呈现出新兴的耐药性.
- 升高的雌激酶活性和特定的通道基因突变是德尔塔美林耐药性的关键机制.
- RmABCC2基因可能在Rhipicephalus microplus.中对ivermectin耐药性起作用.
- 持续监测和综合性害虫管理策略对于可持续的控制至关重要.
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